Integrative functional genetic-epigenetic approach for selecting genes as urine biomarkers for bladder cancer diagnosis

Early screening for bladder cancer (BC) holds the key to combat and control the increasing global burden of BC mortality. We presented a simple approach to characterize, analyze, and validate a panel of biomarkers in BC and their relationship to bilharziasis. We investigated voided urine and blood s...

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Bibliographic Details
Published in:Tumor biology 2015-12, Vol.36 (12), p.9545-9552
Main Authors: Eissa, Sanaa, Matboli, Marwa, Essawy, Nada O. E., Kotb, Youssef M.
Format: Article
Language:English
Subjects:
Aged
Biomarkers
Biomarkers, Tumor - urine
Biomedical and Life Sciences
Biomedicine
Bladder cancer
Cancer Research
Epigenesis, Genetic - genetics
Epigenetics
Female
Genes
Humans
Hyaluronoglucosaminidase - urine
Male
Medical diagnosis
MicroRNAs - urine
Middle Aged
Research Article
RNA, Long Noncoding - urine
Schistosomiasis - pathology
Schistosomiasis - urine
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - pathology
Urinary Bladder Neoplasms - urine
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Summary:Early screening for bladder cancer (BC) holds the key to combat and control the increasing global burden of BC mortality. We presented a simple approach to characterize, analyze, and validate a panel of biomarkers in BC and their relationship to bilharziasis. We investigated voided urine and blood samples from patients with bladder cancer ( n  = 94), benign bladder lesions ( n  = 60), and age-matched normal controls ( n  = 56). This study was divided into the following phases. (1) We analyzed the expression of urinary Hyaluronoglucosaminidase 1 (HYAL1) protein in BC and control samples by zymography. (2) We performed bioinformatics analysis to retrieve a set of epigenetic regulators of HYAL1. (3) This set of three selected genes [long non-coding RNA-urothelial cancer associated 1( lncRNA -UCA1), microRNA-210, and microRNA-96] was then analyzed in the same urine samples used in phase I by quantitative real-time PCR. (4) A high reproducibility of gene selection results was also determined from statistical validation. The urinary expression of HYAL1 protein and its epigenetic regulators were higher in BC patients ( P  
ISSN:1010-4283
1423-0380
DOI:10.1007/s13277-015-3722-6