Bacterial Diversity in the Soda Saline Crater Lake from Isabel Island, Mexico

• Published in: Microbial ecology 2016-01, Vol.71 (1), p.68-77
• Main Authors: Aguirre-Garrido, José Félix, Ramírez-Saad, Hugo César, Toro, Nicolás, Martínez-Abarca, Francisco
• Format: Article
• Language: English
• Subjects: Alcanivorax; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; bacterial communities; Biodiversity; Biomedical and Life Sciences; Biota; birds; coasts; Cyanobacteria; Cyclobacterium; denaturing gradient gel electrophoresis; Desulfofustis; Desulfotignum; Desulfuromonas; Ecology; ecosystems; ENVIRONMENTAL MICROBIOLOGY; Evaporation; excretion; Flavobacteriaceae; Freshwater; genes; Geoecology/Natural Processes; Halomonas; heterotrophs; Idiomarina; Islands; lakes; Lakes - analysis; Lakes - microbiology; Life Sciences; Meromictic lakes; Mexico; Microbial Ecology; Microbiology; Microorganisms; Molecular Sequence Data; Nature Conservation; nutrients; Phylogeny; prokaryotic cells; rain; ribosomal RNA; Salegentibacter; salt tolerance; Sediment samplers; sediments; sequence analysis; Sodium Chloride - analysis; Sodium Chloride - metabolism; species diversity; Sulfur; Sulfur cycle; Water Quality/Water Pollution
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-015-0676-6

Isabel Lake is a moderate saline soda crater lake located in Isabel Island in the eastern tropical Pacific coast of Mexico. Lake is mainly formed by rainfall and is strongly affected by evaporation and high input of nutrients derived from excretions of a large bird community inhabiting the island. So far, only the island macrobiota has been studied. The knowledge of the prokaryotic biota inhabiting the upper layers of this meromictic lake can give clues for the maintenance of this ecosystem. We assessed the diversity and composition of prokaryotic community in sediments and water of the lake by DGGE profiling, 16S rRNA gene amplicon pyrosequencing, and cultivation techniques. The bacterial community is largely dominated by halophilic and halotolerant microorganisms. Alpha diversity estimations reveal higher value in sediments than in water (P > 0.005). The lake water is dominated by γ-Proteobacteria belonging to four main families where Halomonadaceae presents the highest abundance. Aerobic, phototrophic, and halotolerant prokaryotes such as Cyanobacteria GPIIa, Halomonas, Alcanivorax, Idiomarina, and Cyclobacterium genera are commonly found. However, in sediment samples, Formosa, Muricauda, and Salegentibacter genera corresponding to Flavobacteriaceae family accounted for 15–20 % of the diversity. Heterotrophs like those involved in sulfur cycle, Desulfotignum, Desulfuromonas, Desulfofustis, and Desulfopila, appear to play an important role in sediments. Finally, a collection of aerobic halophilic bacterial isolates was created from these samples; members of the genus Halomonas were predominantly isolated from lake water. This study contributes to state the bacterial diversity present in this particular soda saline crater lake.