A novel N-acetyl-glucosamine lectin of Lonchocarpus araripensis attenuates acute cellular inflammation in mice

Objective and design This study had investigated the anti-inflammatory activity of a seed lectin (LAL) isolated from Lonchocarpus araripensis. Material/methods LAL was purified by affinity chromatography (chitin column) and ion exchange chromatography (DEAE-Sephacel). In vitro LAL was tested for hem...

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Bibliographic Details
Published in:Inflammation research 2016-01, Vol.65 (1), p.43-52
Main Authors: Pires, Alana F., Rodrigues, Natália V. F. C., Soares, Pedro Marcos G., Ribeiro, Ronaldo de Albuquerque, Aragão, Karoline S., Marinho, Márcia M., da Silva, Mayara T. L., Cavada, Benildo S., Assreuy, Ana Maria S.
Format: Article
Language:English
Subjects:
Acetylglucosamine - pharmacology
Allergology
Animals
Anti-Inflammatory Agents - pharmacology
Biomedical and Life Sciences
Biomedicine
Capillary Permeability - drug effects
Dermatology
Edema - chemically induced
Edema - prevention & control
Erythrocytes - drug effects
Fabaceae - chemistry
Hemagglutination - drug effects
Immunology
In Vitro Techniques
Inflammation - pathology
Inflammation - prevention & control
Lectins - pharmacology
Lonchocarpus
Male
Mice
Neurology
Original Research Paper
Peritonitis - chemically induced
Peritonitis - prevention & control
Pharmacology/Toxicology
Rabbits
Rheumatology
Seeds - chemistry
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Summary:Objective and design This study had investigated the anti-inflammatory activity of a seed lectin (LAL) isolated from Lonchocarpus araripensis. Material/methods LAL was purified by affinity chromatography (chitin column) and ion exchange chromatography (DEAE-Sephacel). In vitro LAL was tested for hemagglutinating activity against rabbit erythrocytes. In vivo LAL was assessed for the anti-inflammatory activity via intravenous injection (i.v.) in Swiss mice (25–30 g; n  = 6/group) in models of paw edema and peritonitis. Statistical analysis: ANOVA ( p  
ISSN:1023-3830
1420-908X
DOI:10.1007/s00011-015-0889-7