Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803: e0130904

The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that "light" plasma membranes have a high car...

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Bibliographic Details
Published in:PloS one 2015-06, Vol.10 (6)
Main Authors: Zhang, Lifang, Selao, Tiago Toscano, Selstam, Eva, Norling, Birgitta
Format: Article
Language:English
Subjects:
Cyanobacteria
Synechocystis
Online Access:Get full text
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Summary:The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that "light" plasma membranes have a high carotenoid/protein ratio, when compared to "heavier" plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of beta -carotene precursor molecules and echinenone.
ISSN:1932-6203
DOI:10.1371/journal.pone.0130904