Identification of Leishmania strains from Jordan

The enzymatic profiles of 22 Jordanian Leishmania isolates obtained from humans, Psammomys obesus and Phlebotomus papatasi were determined using starch-gel electrophoresis and a 15-enzyme system. Thirteen of the isolates were typed as L. major and the other nine, all from Mediterranean or sub-Medite...

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Bibliographic Details
Published in:Annals of tropical medicine and parasitology 2004-10, Vol.98 (7), p.677-683
Main Authors: Saliba, E.K., Pratlong, F., Dedet, J.P., Saleh, N., Khoury, S.A., Oumeish, O.Y., Batayneh, O., Al-Oran, R.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Disease Reservoirs
Electrophoresis, Starch Gel - methods
General aspects
Gerbillinae - parasitology
Humans
Insect Vectors
Isoenzymes - metabolism
Jordan - epidemiology
Leishmania - classification
Leishmania - enzymology
Leishmania - isolation & purification
Leishmania major - classification
Leishmania major - enzymology
Leishmania major - isolation & purification
Leishmania tropica - classification
Leishmania tropica - enzymology
Leishmania tropica - isolation & purification
Leishmaniasis, Cutaneous - epidemiology
Leishmaniasis, Cutaneous - parasitology
Medical sciences
Phlebotomus - parasitology
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Summary:The enzymatic profiles of 22 Jordanian Leishmania isolates obtained from humans, Psammomys obesus and Phlebotomus papatasi were determined using starch-gel electrophoresis and a 15-enzyme system. Thirteen of the isolates were typed as L. major and the other nine, all from Mediterranean or sub-Mediterranean regions, as L. tropica. The two zymodemes of L. major encountered, MON-26 and MON-103, differed in terms of purine nucleoside phosphorylase 2. The MON-26 isolates came from the Jordanian plateau whereas those of MON-103 were only collected from the Jordan valley. The four zymodemes of L. tropica observed (MON-7, MON-137, MON-200 and MON-265) were identical for only two of the 15 enzymes studied (i.e. isocitrate dehydrogenase and glucose phosphate isomerase), confirming the high level of enzymatic polymorphism of L. tropica. So far, MON-200 and MON-265 have only been found in Jordan.
ISSN:0003-4983
1364-8594
DOI:10.1179/000349804X3144