Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development

Secreted mucins constitute a crucial part of the gel that protects respiratory and digestive epithelia, being MUC2/Muc2 the predominant gel-forming mucin of the intestine while MUC5AC/Muc5ac is one of the gel-forming mucins most expressed at the airways. In this study, we have analyzed Muc2 and Muc5...

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Bibliographic Details
Published in:Research in veterinary science 2016-02, Vol.104, p.136-145
Main Authors: Ferretti, V.A., Segal-Eiras, A., Barbeito, C.G., Croce, M.V.
Format: Article
Language:English
Subjects:
Animals
Development
Embryo, Mammalian - metabolism
Embryology
Embryonic Development
Fetuses
Gastrointestinal Tract - growth & development
Gastrointestinal Tract - metabolism
Gene Expression
Laboratory animals
Molecular weight
Muc2
Muc5ac
Mucin 5AC - genetics
Mucin 5AC - metabolism
Mucin-2 - genetics
Mucin-2 - metabolism
Rat
Rats - genetics
Rats - growth & development
Rats - metabolism
Respiratory System - growth & development
Respiratory System - metabolism
Veterinary medicine
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Summary:Secreted mucins constitute a crucial part of the gel that protects respiratory and digestive epithelia, being MUC2/Muc2 the predominant gel-forming mucin of the intestine while MUC5AC/Muc5ac is one of the gel-forming mucins most expressed at the airways. In this study, we have analyzed Muc2 and Muc5ac during rat development by using immunohistochemistry, Western blotting and RT-PCR. We demonstrated that rat Muc2 was expressed in fetal intestinal goblet cells of surface epithelium of villi and developing Lieberkühn crypts. In neonates and adults, Muc2 was expressed at luminal goblet cells of small and large intestine and at gastric mucous and glandular cells. Muc5ac protein was observed in embryonic gastric and lung samples; expression increased during development and postnatal and adult life. After birth, a low reaction was detected at the tracheal surface epithelium and glands, which increased in adults. •Muc2 and Muc5ac form the mucus layer of gastrointestinal and airway adult tracts.•Muc2 and Muc5ac expression of prenatal, perinatal and adult rat was studied.•Muc2 was detected on emergent goblet cells and developing Lieberkühn glands.•Muc5ac was found early during gastric and lung embryonic development.•These mucins may contribute with respiratory and gastrointestinal development.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2015.12.012