Multicentre evaluation of a real-time PCR assay to detect genes encoding clinically relevant carbapenemases in cultured bacteria

Highlights • Rapid and accurate detection of acquired carbapenemases aids effective patient management. • Real-time PCR identified the target genes in 502 isolates in a central laboratory. • The target genes were accurately identified in 100 isolates distributed to five different centres. • In >1...

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Bibliographic Details
Published in:International journal of antimicrobial agents 2016-02, Vol.47 (2), p.151-154
Main Authors: Ellington, Matthew J, Findlay, Jacqueline, Hopkins, Katie L, Meunier, Danièle, Alvarez-Buylla, Adela, Horner, Carolyne, McEwan, Ashley, Guiver, Malcolm, McCrae, Li-Xu, Woodford, Neil, Hawkey, Peter
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
beta-Lactamases - genetics
Enterobacteriaceae
Gram-Negative Bacteria - enzymology
Gram-Negative Bacteria - genetics
Humans
Infectious Disease
KPC
Metallo-β-lactamase
Multiplex Polymerase Chain Reaction - methods
NDM
OXA-48
Real-Time Polymerase Chain Reaction - methods
Sensitivity and Specificity
VIM
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Summary:Highlights • Rapid and accurate detection of acquired carbapenemases aids effective patient management. • Real-time PCR identified the target genes in 502 isolates in a central laboratory. • The target genes were accurately identified in 100 isolates distributed to five different centres. • In >1000 tests across five different centres, the assay performed with 100% sensitivity and specificity. • The assay is portable and reliable for the detection KPC, NDM, VIM and OXA-48-like carbapenemases.
ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2015.11.013