HELB Is a Feedback Inhibitor of DNA End Resection

• Published in: Molecular cell 2016-02, Vol.61 (3), p.405-418
• Main Authors: Tkáč, Ján, Xu, Guotai, Adhikary, Hemanta, Young, Jordan T.F., Gallo, David, Escribano-Díaz, Cristina, Krietsch, Jana, Orthwein, Alexandre, Munro, Meagan, Sol, Wendy, Al-Hakim, Abdallah, Lin, Zhen-Yuan, Jonkers, Jos, Borst, Piet, Brown, Grant W., Gingras, Anne-Claude, Rottenberg, Sven, Masson, Jean-Yves, Durocher, Daniel
• Format: Article
• Language: English
• Subjects: Animals; BRCA1 Protein - genetics; DNA End-Joining Repair; DNA Helicases - deficiency; DNA Helicases - genetics; DNA Helicases - metabolism; DNA Repair Enzymes - genetics; DNA Repair Enzymes - metabolism; Exodeoxyribonucleases - genetics; Exodeoxyribonucleases - metabolism; Feedback, Physiological; Female; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; HEK293 Cells; HeLa Cells; Humans; Mammary Neoplasms, Experimental - drug therapy; Mammary Neoplasms, Experimental - enzymology; Mammary Neoplasms, Experimental - genetics; Mammary Neoplasms, Experimental - pathology; Mice; Mice, 129 Strain; Mice, Inbred C57BL; Mice, Knockout; Phthalazines - pharmacology; Piperazines - pharmacology; Poly(ADP-ribose) Polymerase Inhibitors - pharmacology; RecQ Helicases - genetics; RecQ Helicases - metabolism; RNA Interference; S Phase; Time Factors; Transfection; Tumor Suppressor Proteins - genetics
• ISSN: 1097-2765; 1097-4164
• DOI: 10.1016/j.molcel.2015.12.013

DNA double-strand break repair by homologous recombination is initiated by the formation of 3′ single-stranded DNA (ssDNA) overhangs by a process termed end resection. Although much focus has been given to the decision to initiate resection, little is known of the mechanisms that regulate the ongoing formation of ssDNA tails. Here we report that DNA helicase B (HELB) underpins a feedback inhibition mechanism that curtails resection. HELB is recruited to ssDNA by interacting with RPA and uses its 5′-3′ ssDNA translocase activity to inhibit EXO1 and BLM-DNA2, the nucleases catalyzing resection. HELB acts independently of 53BP1 and is exported from the nucleus as cells approach S phase, concomitant with the upregulation of resection. Consistent with its role as a resection antagonist, loss of HELB results in PARP inhibitor resistance in BRCA1-deficient tumor cells. We conclude that mammalian DNA end resection triggers its own inhibition via the recruitment of HELB. [Display omitted] •HELB is recruited to resected DSB ends via an interaction with RPA•HELB uses its 5′-3′ translocase activity to inhibit DNA end resection•Nuclear export of HELB participates in the activation of resection in S phase•HELB loss leads to PARPi resistance in BRCA1-deficient tumor cells Tkáč et al. report that HELB underpins a feedback inhibition mechanism that curtails mammalian DNA end resection. HELB is recruited to ssDNA in an RPA-dependent manner, where it limits long-range resection by the BLM-DNA2 and EXO1 nuclease machineries. Consequently, HELB is critical for the PARP inhibitor sensitivity of BRCA1-deficient tumors.