Function of GATA Transcription Factors in Preadipocyte-Adipocyte Transition

• Published in: Science (American Association for the Advancement of Science) 2000-10, Vol.290 (5489), p.134-138
• Main Authors: Tong, Qiang, Dalgin, Gökhan, Xu, Haiyan, Ting, Chao-Nan, Leiden, Jeffrey M., Hotamisligil, Gökhan S.
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Adipocytes; Adipocytes - cytology; Adipocytes - metabolism; adipogenesis; Adipose Tissue - cytology; Adipose Tissue - metabolism; Adipose Tissue, Brown - cytology; Adipose Tissue, Brown - metabolism; Animals; Biological and medical sciences; Body fat; Cell Differentiation; Cells, Cultured; Cellular biology; Cellular differentiation; DNA; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Embryonic stem cells; Fat cells; Feedback (Response); Fundamental and applied biological sciences. Psychology; GATA transcription factors; GATA-2 protein; GATA-3 protein; GATA2 Transcription Factor; GATA3 Transcription Factor; Gene Expression; Genes; Genetic aspects; Genetic markers; Genetics; Individualized Instruction; Lipogenesis; Mice; Molecular and cellular biology; Molecular genetics; Mutation; Obesity; Obesity - genetics; Obesity - metabolism; peroxisome proliferator-activated receptor ^g; Pluripotent stem cells; Promoter Regions, Genetic; Receptors; Receptors, Cytoplasmic and Nuclear - genetics; Receptors, Cytoplasmic and Nuclear - metabolism; Stem cells; Stem Cells - cytology; Trans-Activators - chemistry; Trans-Activators - genetics; Trans-Activators - metabolism; Transcription Factors - chemistry; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Universities and colleges; University research; Zinc Fingers
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.290.5489.134

Genes that control the early stages of adipogenesis remain largely unknown. Here, we show that murine GATA-2 and GATA-3 are specifically expressed in white adipocyte precursors and that their down-regulation sets the stage for terminal differentiation. Constitutive GATA-2 and GATA-3 expression suppressed adipocyte differentiation and trapped cells at the preadipocyte stage. This effect is mediated, at least in part, through the direct suppression of peroxisome proliferator-activated receptor γ. GATA-3-deficient embryonic stem cells exhibit an enhanced capacity to differentiate into adipocytes, and defective GATA-2 and GATA-3 expression is associated with obesity. Thus, GATA-2 and GATA-3 regulate adipocyte differentiation through molecular control of the preadipocyte-adipocyte transition.