A STRESS-INDUCED PROTEIN ASSOCIATED WITH THE GIRDLE BAND REGION OF THE DIATOM THALASSIOSIRA PSEUDONANA (BACILLARIOPHYTA)

We report the characterization of a cell-surface protein isolated from the centric diatom Thalassiosira pseudonana Hasle and Heimdal. This protein has an apparent molecular weight of 150 kDa, is highly acidic, and is intimately associated with the cell wall. Although originally identified in cells e...

Full description

Saved in:
Bibliographic Details
Published in:Journal of phycology 2005-06, Vol.41 (3), p.577-589
Main Authors: Davis, Aubrey K., Hildebrand, Mark, Palenik, Brian
Format: Article
Language:English
Subjects:
Bacillariophyta
cell cycle
cell surface
chitin binding domains
copper
diatom
girdle band
iron
Marine
silicon
stress
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 589
container_issue 3
container_start_page 577
container_title Journal of phycology
container_volume 41
creator Davis, Aubrey K.
Hildebrand, Mark
Palenik, Brian
description We report the characterization of a cell-surface protein isolated from the centric diatom Thalassiosira pseudonana Hasle and Heimdal. This protein has an apparent molecular weight of 150 kDa, is highly acidic, and is intimately associated with the cell wall. Although originally identified in cells experiencing copper toxicity, it is also induced by silicon and iron limitation but not by phosphate or nitrate limitation. Using immunofluorescence techniques, the 150-kDa protein was localized to the girdle band region and covered the elongated girdle band region of morphologically aberrant cells suffering from copper toxicity. Although having biochemical similarities to girdle band associated proteins identified in pennate diatoms known as pleuralins, the 150-kDa protein is not a sequence homolog and is predicted to have a number of unique features, such as chitin binding domains and a possible RGD cell attachment motif. Results presented here suggest that this protein is normally cell cycle regulated and may be involved in stabilizing cells during the division process.
doi_str_mv 10.1111/j.1529-8817.2005.00076.x
format article
fullrecord <record><control><sourceid>proquest_istex</sourceid><recordid>TN_cdi_proquest_miscellaneous_17631648</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17631648</sourcerecordid><originalsourceid>FETCH-LOGICAL-i224t-c5169783ac3c0be1870aac942535592c4e0cb0356c74ecb9fea6353ee9c8650e3</originalsourceid><addsrcrecordid>eNo9jF1PgzAUhhujiXP6H3pl9ALsBy1w2UEHjUgXYFn0hrCmS5ibm7Al899LNuN7c07O85wXAIiRi4e8rF3MSOgEAfZdghBzEUI-d09XYPQPrsEIIUIcyj1-C-76fn2WGB6Bk4BlVciydFQezyMZw1mhK6lyKMpSR0pUw2mhqhRWqYSJKuJMwonIY1jIROkc6umZxIOp34ZVZMOj0qUqBJyVch7rXOQCPk1EpLJMFErP0vdKPN-Dm1Wz6e3D3xyD-VRWUepkOlGRyJyWEO_gGIZ56Ae0MdSgpcWBj5rGhB5hlLGQGM8is0SUceN71izDlW04ZdTa0AScIUvH4PHSu-9230fbH-pt2xu72TRfdnfsa-xzirkXDKJzEdv-YE_1vmu3TfdTN91nzX3qs3qRJ_XCm9BX76OsI_oLJO5mRw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17631648</pqid></control><display><type>article</type><title>A STRESS-INDUCED PROTEIN ASSOCIATED WITH THE GIRDLE BAND REGION OF THE DIATOM THALASSIOSIRA PSEUDONANA (BACILLARIOPHYTA)</title><source>Wiley-Blackwell Read &amp; Publish Collection</source><creator>Davis, Aubrey K. ; Hildebrand, Mark ; Palenik, Brian</creator><creatorcontrib>Davis, Aubrey K. ; Hildebrand, Mark ; Palenik, Brian</creatorcontrib><description>We report the characterization of a cell-surface protein isolated from the centric diatom Thalassiosira pseudonana Hasle and Heimdal. This protein has an apparent molecular weight of 150 kDa, is highly acidic, and is intimately associated with the cell wall. Although originally identified in cells experiencing copper toxicity, it is also induced by silicon and iron limitation but not by phosphate or nitrate limitation. Using immunofluorescence techniques, the 150-kDa protein was localized to the girdle band region and covered the elongated girdle band region of morphologically aberrant cells suffering from copper toxicity. Although having biochemical similarities to girdle band associated proteins identified in pennate diatoms known as pleuralins, the 150-kDa protein is not a sequence homolog and is predicted to have a number of unique features, such as chitin binding domains and a possible RGD cell attachment motif. Results presented here suggest that this protein is normally cell cycle regulated and may be involved in stabilizing cells during the division process.</description><identifier>ISSN: 0022-3646</identifier><identifier>EISSN: 1529-8817</identifier><identifier>DOI: 10.1111/j.1529-8817.2005.00076.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Inc</publisher><subject>Bacillariophyta ; cell cycle ; cell surface ; chitin binding domains ; copper ; diatom ; girdle band ; iron ; Marine ; silicon ; stress</subject><ispartof>Journal of phycology, 2005-06, Vol.41 (3), p.577-589</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Davis, Aubrey K.</creatorcontrib><creatorcontrib>Hildebrand, Mark</creatorcontrib><creatorcontrib>Palenik, Brian</creatorcontrib><title>A STRESS-INDUCED PROTEIN ASSOCIATED WITH THE GIRDLE BAND REGION OF THE DIATOM THALASSIOSIRA PSEUDONANA (BACILLARIOPHYTA)</title><title>Journal of phycology</title><description>We report the characterization of a cell-surface protein isolated from the centric diatom Thalassiosira pseudonana Hasle and Heimdal. This protein has an apparent molecular weight of 150 kDa, is highly acidic, and is intimately associated with the cell wall. Although originally identified in cells experiencing copper toxicity, it is also induced by silicon and iron limitation but not by phosphate or nitrate limitation. Using immunofluorescence techniques, the 150-kDa protein was localized to the girdle band region and covered the elongated girdle band region of morphologically aberrant cells suffering from copper toxicity. Although having biochemical similarities to girdle band associated proteins identified in pennate diatoms known as pleuralins, the 150-kDa protein is not a sequence homolog and is predicted to have a number of unique features, such as chitin binding domains and a possible RGD cell attachment motif. Results presented here suggest that this protein is normally cell cycle regulated and may be involved in stabilizing cells during the division process.</description><subject>Bacillariophyta</subject><subject>cell cycle</subject><subject>cell surface</subject><subject>chitin binding domains</subject><subject>copper</subject><subject>diatom</subject><subject>girdle band</subject><subject>iron</subject><subject>Marine</subject><subject>silicon</subject><subject>stress</subject><issn>0022-3646</issn><issn>1529-8817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNo9jF1PgzAUhhujiXP6H3pl9ALsBy1w2UEHjUgXYFn0hrCmS5ibm7Al899LNuN7c07O85wXAIiRi4e8rF3MSOgEAfZdghBzEUI-d09XYPQPrsEIIUIcyj1-C-76fn2WGB6Bk4BlVciydFQezyMZw1mhK6lyKMpSR0pUw2mhqhRWqYSJKuJMwonIY1jIROkc6umZxIOp34ZVZMOj0qUqBJyVch7rXOQCPk1EpLJMFErP0vdKPN-Dm1Wz6e3D3xyD-VRWUepkOlGRyJyWEO_gGIZ56Ae0MdSgpcWBj5rGhB5hlLGQGM8is0SUceN71izDlW04ZdTa0AScIUvH4PHSu-9230fbH-pt2xu72TRfdnfsa-xzirkXDKJzEdv-YE_1vmu3TfdTN91nzX3qs3qRJ_XCm9BX76OsI_oLJO5mRw</recordid><startdate>200506</startdate><enddate>200506</enddate><creator>Davis, Aubrey K.</creator><creator>Hildebrand, Mark</creator><creator>Palenik, Brian</creator><general>Blackwell Science Inc</general><scope>BSCLL</scope><scope>7TN</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>200506</creationdate><title>A STRESS-INDUCED PROTEIN ASSOCIATED WITH THE GIRDLE BAND REGION OF THE DIATOM THALASSIOSIRA PSEUDONANA (BACILLARIOPHYTA)</title><author>Davis, Aubrey K. ; Hildebrand, Mark ; Palenik, Brian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i224t-c5169783ac3c0be1870aac942535592c4e0cb0356c74ecb9fea6353ee9c8650e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Bacillariophyta</topic><topic>cell cycle</topic><topic>cell surface</topic><topic>chitin binding domains</topic><topic>copper</topic><topic>diatom</topic><topic>girdle band</topic><topic>iron</topic><topic>Marine</topic><topic>silicon</topic><topic>stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Davis, Aubrey K.</creatorcontrib><creatorcontrib>Hildebrand, Mark</creatorcontrib><creatorcontrib>Palenik, Brian</creatorcontrib><collection>Istex</collection><collection>Oceanic Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 3: Aquatic Pollution &amp; Environmental Quality</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Journal of phycology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Davis, Aubrey K.</au><au>Hildebrand, Mark</au><au>Palenik, Brian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A STRESS-INDUCED PROTEIN ASSOCIATED WITH THE GIRDLE BAND REGION OF THE DIATOM THALASSIOSIRA PSEUDONANA (BACILLARIOPHYTA)</atitle><jtitle>Journal of phycology</jtitle><date>2005-06</date><risdate>2005</risdate><volume>41</volume><issue>3</issue><spage>577</spage><epage>589</epage><pages>577-589</pages><issn>0022-3646</issn><eissn>1529-8817</eissn><abstract>We report the characterization of a cell-surface protein isolated from the centric diatom Thalassiosira pseudonana Hasle and Heimdal. This protein has an apparent molecular weight of 150 kDa, is highly acidic, and is intimately associated with the cell wall. Although originally identified in cells experiencing copper toxicity, it is also induced by silicon and iron limitation but not by phosphate or nitrate limitation. Using immunofluorescence techniques, the 150-kDa protein was localized to the girdle band region and covered the elongated girdle band region of morphologically aberrant cells suffering from copper toxicity. Although having biochemical similarities to girdle band associated proteins identified in pennate diatoms known as pleuralins, the 150-kDa protein is not a sequence homolog and is predicted to have a number of unique features, such as chitin binding domains and a possible RGD cell attachment motif. Results presented here suggest that this protein is normally cell cycle regulated and may be involved in stabilizing cells during the division process.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Inc</pub><doi>10.1111/j.1529-8817.2005.00076.x</doi><tpages>13</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0022-3646
ispartof Journal of phycology, 2005-06, Vol.41 (3), p.577-589
issn 0022-3646
1529-8817
language eng
recordid cdi_proquest_miscellaneous_17631648
source Wiley-Blackwell Read & Publish Collection
subjects Bacillariophyta
cell cycle
cell surface
chitin binding domains
copper
diatom
girdle band
iron
Marine
silicon
stress
title A STRESS-INDUCED PROTEIN ASSOCIATED WITH THE GIRDLE BAND REGION OF THE DIATOM THALASSIOSIRA PSEUDONANA (BACILLARIOPHYTA)
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T12%3A00%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_istex&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20STRESS-INDUCED%20PROTEIN%20ASSOCIATED%20WITH%20THE%20GIRDLE%20BAND%20REGION%20OF%20THE%20DIATOM%20THALASSIOSIRA%20PSEUDONANA%20(BACILLARIOPHYTA)&rft.jtitle=Journal%20of%20phycology&rft.au=Davis,%20Aubrey%20K.&rft.date=2005-06&rft.volume=41&rft.issue=3&rft.spage=577&rft.epage=589&rft.pages=577-589&rft.issn=0022-3646&rft.eissn=1529-8817&rft_id=info:doi/10.1111/j.1529-8817.2005.00076.x&rft_dat=%3Cproquest_istex%3E17631648%3C/proquest_istex%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-i224t-c5169783ac3c0be1870aac942535592c4e0cb0356c74ecb9fea6353ee9c8650e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17631648&rft_id=info:pmid/&rfr_iscdi=true