Use of a feedback-insensitive α subunit of anthranilate synthase as a selectable marker for transformation of rice and potato

A selection system based on a mutant rice gene for a feedback-insensitive α subunit of anthranilate synthase (OASA1D) was developed for the transformation of rice and potato. Expression of OASA1D conferred resistance to the tryptophan analog 5-methyltryptophan (5MT) in transformed cells of rice and...

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Bibliographic Details
Published in:Molecular breeding 2005-01, Vol.14 (4), p.363-373
Main Authors: Yamada, Tetsuya, Tozawa, Yuzuru, Hasegawa, Hisakazu, Terakawa, Teruhiko, Ohkawa, Yasunobu, Wakasa, Kyo
Format: Article
Language:English
Subjects:
Anthranilate synthase
Cell culture
Feedback
Genetic transformation
Hygromycin
Hygromycin B
Kanamycin
Molecular biology
Morphology
Oryza
Plant biology
Plant species
Potatoes
Rice
Transformed cells
Transgenic plants
Tryptophan
Vegetables
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Summary:A selection system based on a mutant rice gene for a feedback-insensitive α subunit of anthranilate synthase (OASA1D) was developed for the transformation of rice and potato. Expression of OASA1D conferred resistance to the tryptophan analog 5-methyltryptophan (5MT) in transformed cells of rice and potato. The selection system based on OASA1D and 5MT was associated with a high transformation efficiency, a short time frame for the generation of transgenic plants, simple culture procedures, and it was as effective as hygromycin B selection in rice (monocotyledon) and kanamycin selection in potato (dicotyledon). Transgenic rice and potato plants established by 5MT selection had normal morphology and accumulated tryptophan when OASA1D was expressed under the control of a constitutive promoter. These results demonstrate the efficacy of OASA1D as a selectable marker and they suggest that the 5MT selection system based on this gene will prove applicable to a wide range of plant species and culture procedures.
ISSN:1380-3743
1572-9788
DOI:10.1007/s11032-005-0184-3