A Common Core RNP Structure Shared between the Small Nucleolar Box C/D RNPs and the Spliceosomal U4 snRNP

The box C/D snoRNAs function in directing 2'-O-methylation and/or as chaperones in the processing of ribosomal RNA. We show here that Snu13p (15.5kD in human), a component of the U4/U6.U5 tri-snRNP, is also associated with the box C/D snoRNAs. Indeed, genetic depletion of Snu13p in yeast leads...

Full description

Saved in:
Bibliographic Details
Published in:Cell 2000-10, Vol.103 (3), p.457-466
Main Authors: Watkins, N J, Segault, V, Charpentier, B, Nottrott, S, Fabrizio, P, Bachi, A, Wilm, M, Rosbash, M, Branlant, C, Luehrmann, R
Format: Article
Language:English
Subjects:
snoRNA
snRNA U4
Snu13 protein
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The box C/D snoRNAs function in directing 2'-O-methylation and/or as chaperones in the processing of ribosomal RNA. We show here that Snu13p (15.5kD in human), a component of the U4/U6.U5 tri-snRNP, is also associated with the box C/D snoRNAs. Indeed, genetic depletion of Snu13p in yeast leads to a major defect in RNA metabolism. The box C/D motif can be folded into a stem-internal loop-stem structure, almost identical to the 15.5kD binding site in the U4 snRNA. Consistent with this, the box C/D motif binds Snu13p/15.5kD in vitro. The similarities in structure and function observed between the U4 snRNP (chaperone for U6) and the box C/D snoRNPs raises the interesting possibility that these particles may have evolved from a common ancestral RNP.
ISSN:0092-8674