Practical and low cost whole-organism motility assay: A step-by-step protocol

Here, we provide a step-by-step protocol for a practical and low cost whole-organism assay for the screening of chemical compounds for activity against parasitic worms. This assay has considerable advantages over conventional methods, mainly in relation to ease of use, throughput, time and cost. It...

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Bibliographic Details
Published in:Molecular and cellular probes 2016-02, Vol.30 (1), p.13-17
Main Authors: Preston, Sarah, Jabbar, Abdul, Nowell, Cameron, Joachim, Anja, Ruttkowski, Baerbel, Cardno, Tony, Hofmann, Andreas, Gasser, Robin B.
Format: Article
Language:English
Subjects:
Animals
Antinematodal Agents - isolation & purification
Antinematodal Agents - pharmacology
Cost-Benefit Analysis
Drug Evaluation, Preclinical - economics
Drug Evaluation, Preclinical - instrumentation
Drug Evaluation, Preclinical - methods
Drug screening
High-Throughput Screening Assays - economics
High-Throughput Screening Assays - instrumentation
High-Throughput Screening Assays - methods
Larva - drug effects
Larva - physiology
Locomotion - drug effects
Locomotion - physiology
Motility assay
Nematode
Parasite
Reproducibility of Results
Step-by-step protocol
Strongylida - drug effects
Strongylida - physiology
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Summary:Here, we provide a step-by-step protocol for a practical and low cost whole-organism assay for the screening of chemical compounds for activity against parasitic worms. This assay has considerable advantages over conventional methods, mainly in relation to ease of use, throughput, time and cost. It is readily suited to the screening of hundreds to thousands of compounds for subsequent hit-to-lead optimisation, and should be applicable to many different parasites and other organisms commensurate with the size of wells in the microtiter plates used for phenotypic screening.
ISSN:0890-8508
1096-1194
DOI:10.1016/j.mcp.2015.08.005