Kv1.3 potassium channel mediates macrophage migration in atherosclerosis by regulating ERK activity

Ion channels expressed in macrophages have been tightly related to atherosclerosis by coupling cellular function. How the voltage-gated potassium channels (Kv) affect macrophage migration remain unknown. The aim of our study is to investigate whether Kv1.3-ERK signaling pathway plays an important ro...

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Bibliographic Details
Published in:Archives of biochemistry and biophysics 2016-02, Vol.591, p.150-156
Main Authors: Kan, Xiao-Hong, Gao, Hai-Qing, Ma, Zhi-Yong, Liu, Lin, Ling, Ming-Ying, Wang, Yuan-Yuan
Format: Article
Language:English
Subjects:
Animals
Atherosclerosis
Cell migration
Cell Movement
Cells, Cultured
Coronary Artery Disease - metabolism
Coronary Artery Disease - pathology
Extracellular signal-regulated kinase
Humans
Kv1.3 Potassium Channel - metabolism
Macrophage
Macrophages - metabolism
MAP Kinase Signaling System
Mice
RAW 264.7 Cells
Voltage-gated potassium channel
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Summary:Ion channels expressed in macrophages have been tightly related to atherosclerosis by coupling cellular function. How the voltage-gated potassium channels (Kv) affect macrophage migration remain unknown. The aim of our study is to investigate whether Kv1.3-ERK signaling pathway plays an important role in the process. We explored the expression of Kv1.3 in coronary atherosclerotic heart disease and found Kv1.3 channel was increased in acute coronary syndrome patients. Treatment of RAW264.7 cells with Kv1.3 small interfering RNA, suppressed cell migration. The expression of phosphorylated ERK1/2 also decreased after knockdown of Kv1.3. On the other hand, overexpression of Kv1.3 channel promoted cell migration and ERK1/2 phosphorylation. U-0126, the mitogen-activated protein kinase inhibitors, could reverse macrophage migration induced by Kv1.3 channel overexpression. Downregulation of Kv1.3 channel by siRNA could not further inhibit cell migration when cells were treated with U-0126. It means that ERK is downstream signal of Kv1.3 channel. We concluded that Kv1.3 may stimulate macrophage migration through the activation of ERK. •Kv1.3 expression is increased in acute coronary syndrome.•Kv1.3 channels promote macrophages migration.•Kv1.3 channels influence cell migration by activating ERK.
ISSN:0003-9861
1096-0384
DOI:10.1016/j.abb.2015.12.013