set of chloroplast microsatellite primers for Eucalyptus (Myrtaceae)

We present a set of 35 chloroplast microsatellite primers for Eucalyptus. Ten of the microsatellites displayed intraspecific polymorphism, and identified nine haplotypes among 16 Eucalyptus globulus individuals. Primer conservation was high, with a polymerase chain reaction (PCR) success rate of 98%...

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Bibliographic Details
Published in:Molecular ecology notes 2005-09, Vol.5 (3), p.538-541
Main Authors: Steane, D.A, Jones, R.C, Vaillancourt, R.E
Format: Article
Language:English
Subjects:
alleles
alleles per locus
Angophora
chloroplast DNA
Corymbia
DNA primers
Eucalyptus
Eucalyptus globulus
genetic markers
genetic polymorphism
haplotypes
loci
Melaleuca
microsatellite repeats
molecular sequence data
Myrtaceae
nucleotide sequences
plastid
polymerase chain reaction
polymorphic loci
Simple sequence repeat (SSR)
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Summary:We present a set of 35 chloroplast microsatellite primers for Eucalyptus. Ten of the microsatellites displayed intraspecific polymorphism, and identified nine haplotypes among 16 Eucalyptus globulus individuals. Primer conservation was high, with a polymerase chain reaction (PCR) success rate of 98% when tested on four other Eucalyptus species and seven additional myrtaceous genera. Chloroplast microsatellites have applications in phylogeographic studies, fingerprinting and progeny analysis.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.00981.x