Nematode-specific PCR primers for the 18S small subunit rRNA gene

A set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to...

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Bibliographic Details
Published in:Molecular ecology notes 2005-09, Vol.5 (3), p.611-612
Main Authors: FLOYD, ROBIN M., ROGERS, ALEX D., LAMBSHEAD, P. JOHN D., SMITH, CRAIG R.
Format: Article
Language:English
Subjects:
185 ribosomal RNA (SSU)
DNA barcoding
Nematodes
specific amplification
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Description
Summary:A set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no sequences from any other organismal group.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.01009.x