Production of a liquid inoculum/spawn of Agaricus bisporus

The production of a homogeneous liquid culture of mushroom mycelium with a high density of viable inoculum points is a prerequisite for the adaptation of the liquid culture technology to the mushroom spawn production process. Homogenisation proved unsuitable as a technique to produce a morphology of...

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Bibliographic Details
Published in:Biotechnology letters 2000-03, Vol.22 (5), p.351-354
Main Authors: FRIEL, M. T, MCLOUGHLIN, A. J
Format: Article
Language:English
Subjects:
Agaricus bisporus
Agronomy. Soil science and plant productions
Biological and medical sciences
Edible fungi production
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Mushrooms
Shear stress
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Summary:The production of a homogeneous liquid culture of mushroom mycelium with a high density of viable inoculum points is a prerequisite for the adaptation of the liquid culture technology to the mushroom spawn production process. Homogenisation proved unsuitable as a technique to produce a morphology of this nature because of the shear sensitive nature of Agaricus bisporus. To overcome this limitation, a homogeneous culture was produced by exposing culture flasks to alternating periods of shear stress (300 rpm on a shaker table for 60 min day^sup -1^) and recovery (23 h day^sup -1^ under static conditions).[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005616516646