Analysis of chitin at the hyphal tip of Candida albicans using calcofluor white

Staining with calcofluor white (CFW), which is known to bind chitin-rich areas of the cell wall, revealed a difference in the fluorescence intensity at the hyphal tip between Candida albicans hyphal cells that were grown in modified Lee (M-Lee) and SPG media. The fluorescence intensity at the tip in...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-09, Vol.69 (9), p.1798-1801
Main Authors: Watanabe, H.(Osaka City Univ. (Japan)), Azuma, M, Igarashi, K, Ooshima, H
Format: Article
Language:English
Subjects:
AGENT PATHOGENE
Benzenesulfonates - pharmacology
Biological and medical sciences
calcofluor white (CFW)
CANDIDA ALBICANS
Candida albicans - chemistry
CELL STRUCTURE
CHITIN
Chitin - analysis
CHITINE
ESTRUCTURA CELULAR
Fluorescent Dyes - pharmacology
FONGICIDE
Fundamental and applied biological sciences. Psychology
FUNGICIDAS
FUNGICIDES
Hyphae - chemistry
hyphal tip
ORGANISMOS PATOGENOS
osmotic pressure
PATHOGENS
QUITINA
STRUCTURE CELLULAIRE
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Summary:Staining with calcofluor white (CFW), which is known to bind chitin-rich areas of the cell wall, revealed a difference in the fluorescence intensity at the hyphal tip between Candida albicans hyphal cells that were grown in modified Lee (M-Lee) and SPG media. The fluorescence intensity at the tip increased with the addition of salts and sugar to SPG. The chitin levels per dry cell weight in cells grown in modified Lee and SPG with 1.0 M NaCl were also higher than in SPG. These results suggest that chitin synthesis at the tip of C. albicans might be activated by the addition of salts and sugar to a medium.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.69.1798