Involvement of the Helix-Loop-Helix Protein Id-1 in the Glucocorticoid Regulation of Tight Junctions in Mammary Epithelial Cells

• Published in: The Journal of biological chemistry 2000-09, Vol.275 (37), p.28649-28658
• Main Authors: Woo, P L, Cercek, A, Desprez, P Y, Firestone, G L
• Format: Article
• Language: English
• Subjects: Actins - analysis; Animals; beta Catenin; Cytoskeletal Proteins - analysis; dexamethasone; DNA-Binding Proteins - physiology; Epithelial Cells - ultrastructure; Glucocorticoids - pharmacology; Helix-Loop-Helix Motifs; helix-loop-helix proteins; Id-1 protein; Inhibitor of Differentiation Protein 1; Isopropyl Thiogalactoside - pharmacology; Mammary Glands, Animal - cytology; Membrane Proteins - analysis; Mice; Phosphoproteins - analysis; Rabbits; Repressor Proteins; Tight Junctions - drug effects; Tight Junctions - physiology; Trans-Activators; Transcription Factors - physiology; Zonula Occludens-1 Protein
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M910373199

Mammary epithelial cell-cell junctions undergo morphological and structural differentiation during pregnancy and lactation, but little is known about the transcriptional regulators that are involved in this process. In Con8 mammary epithelial tumor cells, we have previously documented that the synthetic glucocorticoid, dexamethasone, induces the reorganization of the tight junction and adherens junction and stimulates the monolayer transepithelial electrical resistance (TER), a reliable in vitro measurement of tight junction sealing. Western blots demonstrated that dexamethasone treatment rapidly and strongly stimulated the level of the Id-1 protein, which is a serum-inducible helix-loop-helix transcriptional repressor. The steroid induction of Id-1 was robust by 4 h of treatment and maintained over a 24-h period. Isopropyl-1-thio-β- d -galactopyranoside-inducible expression of exogenous Id-1 in Con8 cells was shown to strongly facilitate the dexamethasone induction of TER in the absence of serum without altering the dexamethasone-dependent reorganization of ZO-1, β-catenin, or F-actin. Ectopic overexpression of Id-1 in the SCp2 nontumorigenic mammary epithelial cells, which does not undergo complete dexamethasone-dependent tight junction reorganization, enhanced the dexamethasone-induced ZO-1 tight junction localization and stimulated the monolayer TER. Moreover, antisense reduction of Id-1 protein in SCp2 cells prevented the apical junction reorganization and dexamethasone-stimulated TER. Our results implicate Id-1 as acting as a critical regulator of mammary epithelial cell-cell interactions at an early step in the glucocorticoid-dependent signaling pathway that controls tight junction integrity.