Five-day stability of thawed plasma: solvent/detergent-treated plasma comparable with fresh-frozen plasma and plasma frozen within 24 hours

• Published in: Transfusion (Philadelphia, Pa.) Pa.), 2016-02, Vol.56 (2), p.404-409
• Main Authors: Neisser-Svae, Andrea, Trawnicek, Laurenz, Heger, Andrea, Mehta, Trupti, Triulzi, Darrell
• Format: Article
• Language: English
• Subjects: ADAM Proteins - chemistry; ADAM Proteins - metabolism; ADAMTS13 Protein; Blood Preservation; Detergents - chemistry; Factor VII - chemistry; Factor VII - metabolism; Factor VIII - chemistry; Factor VIII - metabolism; Female; Humans; Male; Plasma; Protein S - chemistry; Protein S - metabolism; Protein Stability; Solvents - chemistry; Time Factors
• ISSN: 0041-1132; 1537-2995
• DOI: 10.1111/trf.13356

BACKGROUND Plasma stored refrigerated for up to 5 days after thawing is common practice in many US hospitals. Therefore, clotting factor activities in fresh‐frozen plasma (FFP), plasma frozen within 24 hours (PF24), and solvent/detergent‐treated plasma (SDP), thawed and stored at 1 to 6°C for up to 5 days, were investigated. STUDY DESIGN AND METHODS Five A, B, O, and AB units of FFP, PF24, and SDP were thawed and maintained for 5 days at 1 to 6°C. The activity of factor (F)V, FVII, FVIII, protein S (PS), and ADAMTS13 was determined in each unit at baseline and every 24 hours thereafter for 5 days. RESULTS After thaw, mean values of the variables tested were within the normal range in all three plasma products although, in SDP, FVIII activity was significantly lower (p = 0.0039). After 5 days of storage all factors significantly declined except for ADAMTS13 activity, which was stable. Mean FVIII and ADAMTS13 activity was comparable in all three plasma products and within the normal range, mean FV activity was significantly lower in FFP and PF24 (p