Blockage of K(Ca)3.1 and Kv1.3 channels of the B lymphocyte decreases the inflammatory monocyte chemotaxis

• Published in: International immunopharmacology 2016-02, Vol.31, p.266-271
• Main Authors: Zhang, Shuangxia, Wang, Xianpei, Ju, Chenhui, Zhu, Lijie, Du, Yimei, Gao, Chuanyu
• Format: Article
• Language: English
• Subjects: Animals; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; Calcium - metabolism; Cell Proliferation - drug effects; Cells, Cultured; Chemokine CCL7 - genetics; Chemokine CCL7 - metabolism; Chemotaxis - drug effects; Chemotaxis - immunology; Cnidarian Venoms - pharmacology; Intermediate-Conductance Calcium-Activated Potassium Channels - antagonists & inhibitors; Intermediate-Conductance Calcium-Activated Potassium Channels - physiology; Kv1.3 Potassium Channel - antagonists & inhibitors; Kv1.3 Potassium Channel - physiology; Lymphocyte Activation - drug effects; Mice; Mice, Inbred C57BL; Monocytes - drug effects; Monocytes - immunology; Pyrazoles - pharmacology
• ISSN: 1878-1705
• DOI: 10.1016/j.intimp.2015.12.032

To investigate the effects of Ca(2+) activated potassium channel KCa3.1 and voltage-gated potassium channel Kv1.3 of B lymphocyte on inflammatory monocytes chemotaxis and the potential mechanisms. Thanswell test was used to detect the inflammatory monocyte (Ly-6C(hi)) chemotaxis caused by the B lymphocyte. Enzyme-linked immunosorbent assay (ELISA) was applied to detecting the C-C motif ligand 7 (CCL7) in cultured media. Cell counting kit-8 (CCK) was used to detect the proliferation of B lymphocytes after activation and blockage of both KCa3.1 and Kv1.3 channels. Western blot was used to detect the expression of phosphorylated extracellular signal-regulated kinase (P-ERK) of the B lymphocytes. When activated, B lymphocytes significantly proliferated. After application of KCa3.1 channel-specific inhibitor TRAM-34 and potent Kv1.3 channel inhibitor ShK, both B lymphocytes proliferation and Ly-6C(hi) monocyte chemotaxis were significantly inhibited. The expression of chemotaxis related factor CCL7 decreased remarkably. The opening of KCa3.1 and Kv1.3 channels promote B lymphocyte activation, proliferation and Ly-6C(hi) monocyte chemotaxis. The increase of CCL7 secretion by B lymphocyte may explain the pro migration effects.