Production of rosmarinic acid and salvianolic acid B from callus culture of Salvia miltiorrhiza with cytotoxicity towards acute lymphoblastic leukemia cells

• Published in: Food chemistry 2016-06, Vol.201, p.292-297
• Main Authors: Wu, Ching-Fen, Karioti, Anastasia, Rohr, Doris, Bilia, Anna Rita, Efferth, Thomas
• Format: Article
• Language: English
• Subjects: Benzofurans - chemistry; Callus culture; CCRF-CEM cells; Chromatography, High Pressure Liquid - methods; Cinnamates - chemistry; Depsides - chemistry; Drugs, Chinese Herbal - chemistry; High performance liquid chromatography (HPLC); Plant Extracts - chemistry; Plant Leaves - chemistry; Precursor Cell Lymphoblastic Leukemia-Lymphoma - drug therapy; Rosmarinic Acid; Salvia miltiorrhiza - chemistry; Secondary metabolites
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2016.01.054

•Callus culture of leaf and stem from Salvia miltiorrhiza generated phenolic compounds.•Rosmarinic acid (RA) and salvianolic acid B (Sal B) are identified as the main constituents.•Combination of RA and Sal B exerted cytotoxicity against acute lymphoblastic CCRF-CEM leukemia cells. Salvia miltiorrhiza (SM) Bunge is one of the widely-used Chinese medicinal herbs. In this study, the chemical constituents and anticancer potential of SM stems and leaves were examined with those of respective callus cultures. The callus culture for stem and leaf explants was initiated in modified Murashige and Skoog (MS) medium. Active constituents of respective extracts were analyzed by high performance liquid chromatography coupled with DAD and MS (HPLC–DAD–MS). Rosmarinic acid (RA) and salvianolic acid B (Sal B) were determined to be the main phenolic compounds. Quantitative analyses revealed that callus stem extracts produced higher amount of RA and Sal B (stem RA: 1.27±0.38%; stem Sal B: 0.87±0.20%) than callus leaf did (leaf RA: 0.28±0.02%; leaf Sal B: 0.07±0.03%). Stem and leaf callus extracts exerted cytotoxic effects towards CCRF-CEM cells (stem: 13.1±0.90μg/ml; leaf: 18.1±0.33μg/ml). As expected, stem extract with higher amount of RA and Sal B showed lower IC50 value than leaf extract. These findings suggest the possibility to isolate bioactive constituents with anticancer properties from in vitro callus cultures of stems and leaves of SM.