Dominant Inheritance of Field-Evolved Resistance to Fipronil in Plutella xylostella (Lepidoptera: Plutellidae)

A field-collected strain (HF) of Plutella xylostella (L.) showed 420-fold resistance to fipronil compared with a susceptible laboratory strain (Roth). The HF-R strain, derived from the HF strain by 25 generations of successive selection with fipronil in the laboratory, developed 2,200-fold resistanc...

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Bibliographic Details
Published in:Journal of economic entomology 2016-02, Vol.109 (1), p.334-338
Main Authors: Wang, Xingliang, Wu, Shuwen, Gao, Weiyue, Wu, Yidong
Format: Article
Language:English
Subjects:
Animals
Chromosomes
Female
Fipronil
Fruit flies
GABA receptor
Genetic crosses
Heredity
Homology
inheritance
Insecticide Resistance
INSECTICIDE RESISTANCE AND RESISTANCE MANAGEMENT
Insecticides
Insecticides - pharmacology
Laboratories
Larva - drug effects
Larva - genetics
Larva - growth & development
Male
Moths - drug effects
Moths - genetics
Moths - growth & development
Mutation
Offspring
Pesticides
Plutella xylostella
Pyrazoles - pharmacology
resistance
Sex linkage
Z chromosomes
γ-Aminobutyric acid
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Summary:A field-collected strain (HF) of Plutella xylostella (L.) showed 420-fold resistance to fipronil compared with a susceptible laboratory strain (Roth). The HF-R strain, derived from the HF strain by 25 generations of successive selection with fipronil in the laboratory, developed 2,200-fold resistance to fipronil relative to the Roth strain. The F1 progeny of the reciprocal crosses between HF-R and Roth showed 640-fold (R♀ × S♂) and 1,380-fold (R♂ × S♀) resistance to fipronil, indicating resistance is inherited as an incompletely dominant trait. Analysis of progeny from a backcross (F1♂ × S♀) suggests that resistance is controlled by one major locus. The LC50 of the R♂ × S♀ cross F1 progeny is slightly but significantly higher than that of the R♀ × S♂ cross F1 progeny, suggesting a minor resistance gene on the Z chromosome. Sequence analysis of PxGABARa1 (an Rdl-homologous GABA receptor gene of P. xylostella) from the HF-R strain identified two mutations A282S and A282G (corresponding to the A302S mutation of the Drosophila melanogaster Rdl gene), which have been previously implicated in fipronil resistance in several insect species including P. xylostella. PxGABARa1 was previously mapped to the Z chromosome of P. xylostella. In conclusion, fipronil resistance in the HF-R strain of P. xylostella was incompletely dominant, and controlled by a major autosomal locus and a sex-linked minor gene (PxGABARa1) on the Z chromosome.
ISSN:0022-0493
1938-291X
DOI:10.1093/jee/tov317