Rapid immunodetection of Escherichia coli

A filtration flow-through design was used to develop the rapid immunodetection of Escherichia coli. Polyclonal anti-E. coli IgG was conjugated to small, 0.8 μ Blue latex beads. Cells were mixed with conjugated beads in the presence of anti-E. coli monoclonal IgM. The suspension was then filtered thr...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 2000-04, Vol.22 (7), p.547-550
Main Authors: Arredondo, Alice R, Dorval, Brent L, Klibanov, Alexander M, Lewis, Kim
Format: Article
Language:English
Subjects:
E coli
Escherichia coli
Infections
Latex
nitrocellulose
urinary tract infection
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A filtration flow-through design was used to develop the rapid immunodetection of Escherichia coli. Polyclonal anti-E. coli IgG was conjugated to small, 0.8 μ Blue latex beads. Cells were mixed with conjugated beads in the presence of anti-E. coli monoclonal IgM. The suspension was then filtered through a 5 μ nitrocellulose membrane. The cell-containing complexes were effectively collected on the filter, forming a blue spot. The method produced reliable detection of E. coli at a concentration of 10^sup 5^ cells ml^sup -1^, which is a current benchmark figure for urinary tract infection (UTI) diagnosis.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005616523897