Evaluation of a creosote-based medium for the growth and preparation of a PAH-degrading bacterial community for bioaugmentation

Creosote was evaluated as an inexpensive carbon source for growing inocula of a polycyclic aromatic hydrocarbon (PAH)-degrading bacterial community (community five). Creosote was a poor growth substrate when provided as sole carbon source in a basal salts solution (BSM). Alternatively, peptone, yeas...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2000-04, Vol.24 (4), p.277-284
Main Authors: JUHASZ, A. L, BRITZ, M. L, STANLEY, G. A
Format: Article
Language:English
Subjects:
Aromatic hydrocarbons
Bacteria
Biodegradation
Biodegradation of pollutants
Biological and medical sciences
Biomass
Bioremediation
Biotechnology
Carbon
Carbon sources
creosote
Energy resources
Environment and pollution
Fundamental and applied biological sciences. Psychology
Glucose
Industrial applications and implications. Economical aspects
Laboratories
Life sciences
Microbiology
Microorganisms
Molecular weight
Peptones
Pollutants
Polycyclic aromatic hydrocarbons
Pyrene
Studies
Yeast
Yeasts
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Summary:Creosote was evaluated as an inexpensive carbon source for growing inocula of a polycyclic aromatic hydrocarbon (PAH)-degrading bacterial community (community five). Creosote was a poor growth substrate when provided as sole carbon source in a basal salts solution (BSM). Alternatively, peptone, yeast extract or glucose in BSM supported high growth rates, but community five could not subsequently degrade pyrene. A combination of creosote and yeast extract in BSM (CYEM) supported growth and maintained the pyrene-degrading capacity of community five. Optimum pyrene-degrading activity occurred when the inocula were grown in creosote and yeast extract concentrations of 2ml L^sup -1^ and 1g L^sup -1^ respectively: concentrations outside these values resulted in either low biomass yields or loss of PAH-degrading activity. CYEM-grown community five inocula degraded 250mg L^sup -1^ of pyrene in BSM at a rate comparable to cultures inoculated with community five grown in BSM-pyrene. However, the CYEM-grown community showed a 40% lower rate of PAH degradation in a synthetic PAH mixture compared with pyrene-grown cells and there was an increase in the lag period before the onset of PAH degradation. This appears to reflect a weaker induction of PAH catabolism by CYEM compared to BSM-pyrene. Journal of Industrial Microbiology & Biotechnology (2000) 24, 277-284.[PUBLICATION ABSTRACT]
ISSN:1367-5435
1476-5535
DOI:10.1038/sj.jim.2900819