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Impact of PEG and PEG-b-PAGE modified PLGA on nanoparticle formation, protein loading and release
[Display omitted] The effect of modifying the well-established pharmaceutical polymer PLGA by different PEG-containing block-copolymers on the preparation of ovalbumin (OVA) loaded PLGA nanoparticles (NPs) was studied. The used polymers contained poly(d,l-lactic-co-glycolic acid) (PLGA), polyethylen...
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| Published in: | International journal of pharmaceutics 2016-03, Vol.500 (1-2), p.187-195 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c412t-54d23930df4b93a0612f0cf5b5e2e5591da7511fe0a085d976bfd2235d08db9d3 |
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| cites | cdi_FETCH-LOGICAL-c412t-54d23930df4b93a0612f0cf5b5e2e5591da7511fe0a085d976bfd2235d08db9d3 |
| container_end_page | 195 |
| container_issue | 1-2 |
| container_start_page | 187 |
| container_title | International journal of pharmaceutics |
| container_volume | 500 |
| creator | Rietscher, René Czaplewska, Justyna A. Majdanski, Tobias C. Gottschaldt, Michael Schubert, Ulrich S. Schneider, Marc Lehr, Claus-Michael |
| description | [Display omitted]
The effect of modifying the well-established pharmaceutical polymer PLGA by different PEG-containing block-copolymers on the preparation of ovalbumin (OVA) loaded PLGA nanoparticles (NPs) was studied. The used polymers contained poly(d,l-lactic-co-glycolic acid) (PLGA), polyethylene glycol (PEG) and poly(allyl glycidyl ether) (PAGE) as building blocks. The double emulsion technique yielded spherical NPs in the size range from 170 to 220nm (PDI |
| doi_str_mv | 10.1016/j.ijpharm.2016.01.021 |
| format | article |
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The effect of modifying the well-established pharmaceutical polymer PLGA by different PEG-containing block-copolymers on the preparation of ovalbumin (OVA) loaded PLGA nanoparticles (NPs) was studied. The used polymers contained poly(d,l-lactic-co-glycolic acid) (PLGA), polyethylene glycol (PEG) and poly(allyl glycidyl ether) (PAGE) as building blocks. The double emulsion technique yielded spherical NPs in the size range from 170 to 220nm (PDI<0.15) for all the differently modified polymers, allowing to directly compare protein loading of and release. PEGylation is usually believed to increase the hydrophilic character of produced particles, favoring encapsulation of hydrophilic substances. However, in this study simple PEGylation of PLGA had only a slight effect on protein release. In contrast, incorporating a PAGE block between the PEG and PLGA units, also eventually enabling active targeting introducing a reactive group, led to a significantly higher loading (+25%) and release rate (+100%), compared to PLGA and PEG-b-PLGA NPs.</description><identifier>ISSN: 0378-5173</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/j.ijpharm.2016.01.021</identifier><identifier>PMID: 26784983</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Drug delivery ; Drug Liberation ; Epoxy Compounds - chemistry ; Lactic Acid - chemistry ; Nanoparticles - chemistry ; Ovalbumin ; Ovalbumin - chemistry ; Particle Size ; Poly(allyl glycidyl ether) ; Poly(d,l-lactic-co-glycolic acid) ; Polyethylene glycol ; Polyethylene Glycols - chemistry ; Polyglycolic Acid - chemistry ; Protein delivery ; Surface Properties</subject><ispartof>International journal of pharmaceutics, 2016-03, Vol.500 (1-2), p.187-195</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-54d23930df4b93a0612f0cf5b5e2e5591da7511fe0a085d976bfd2235d08db9d3</citedby><cites>FETCH-LOGICAL-c412t-54d23930df4b93a0612f0cf5b5e2e5591da7511fe0a085d976bfd2235d08db9d3</cites><orcidid>0000-0003-2013-0614</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26784983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rietscher, René</creatorcontrib><creatorcontrib>Czaplewska, Justyna A.</creatorcontrib><creatorcontrib>Majdanski, Tobias C.</creatorcontrib><creatorcontrib>Gottschaldt, Michael</creatorcontrib><creatorcontrib>Schubert, Ulrich S.</creatorcontrib><creatorcontrib>Schneider, Marc</creatorcontrib><creatorcontrib>Lehr, Claus-Michael</creatorcontrib><title>Impact of PEG and PEG-b-PAGE modified PLGA on nanoparticle formation, protein loading and release</title><title>International journal of pharmaceutics</title><addtitle>Int J Pharm</addtitle><description>[Display omitted]
The effect of modifying the well-established pharmaceutical polymer PLGA by different PEG-containing block-copolymers on the preparation of ovalbumin (OVA) loaded PLGA nanoparticles (NPs) was studied. The used polymers contained poly(d,l-lactic-co-glycolic acid) (PLGA), polyethylene glycol (PEG) and poly(allyl glycidyl ether) (PAGE) as building blocks. The double emulsion technique yielded spherical NPs in the size range from 170 to 220nm (PDI<0.15) for all the differently modified polymers, allowing to directly compare protein loading of and release. PEGylation is usually believed to increase the hydrophilic character of produced particles, favoring encapsulation of hydrophilic substances. However, in this study simple PEGylation of PLGA had only a slight effect on protein release. 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The effect of modifying the well-established pharmaceutical polymer PLGA by different PEG-containing block-copolymers on the preparation of ovalbumin (OVA) loaded PLGA nanoparticles (NPs) was studied. The used polymers contained poly(d,l-lactic-co-glycolic acid) (PLGA), polyethylene glycol (PEG) and poly(allyl glycidyl ether) (PAGE) as building blocks. The double emulsion technique yielded spherical NPs in the size range from 170 to 220nm (PDI<0.15) for all the differently modified polymers, allowing to directly compare protein loading of and release. PEGylation is usually believed to increase the hydrophilic character of produced particles, favoring encapsulation of hydrophilic substances. However, in this study simple PEGylation of PLGA had only a slight effect on protein release. In contrast, incorporating a PAGE block between the PEG and PLGA units, also eventually enabling active targeting introducing a reactive group, led to a significantly higher loading (+25%) and release rate (+100%), compared to PLGA and PEG-b-PLGA NPs.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26784983</pmid><doi>10.1016/j.ijpharm.2016.01.021</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-2013-0614</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of pharmaceutics, 2016-03, Vol.500 (1-2), p.187-195 |
| issn | 0378-5173 1873-3476 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Drug delivery Drug Liberation Epoxy Compounds - chemistry Lactic Acid - chemistry Nanoparticles - chemistry Ovalbumin Ovalbumin - chemistry Particle Size Poly(allyl glycidyl ether) Poly(d,l-lactic-co-glycolic acid) Polyethylene glycol Polyethylene Glycols - chemistry Polyglycolic Acid - chemistry Protein delivery Surface Properties |
| title | Impact of PEG and PEG-b-PAGE modified PLGA on nanoparticle formation, protein loading and release |
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