Surface-displayed viral antigens on Salmonella carrier vaccine

We have developed a recombinant live oral vaccine using the ice-nucleation protein (Inp) from Pseudomonas syringae to display viral antigens on the surface of Salmonella spp . Fusion proteins containing viral antigens were expressed in the oral vaccine strain, Salmonella typhi Ty21a. Surface localiz...

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Bibliographic Details
Published in:Nature biotechnology 2000-06, Vol.18 (6), p.645-648
Main Authors: Kim, Chul-Joong, Lee, Jong-Soo, Shin, Kwang-Soon, Pan, Jae-Gu
Format: Article
Language:English
Subjects:
Administration, Intranasal
Agriculture
Animals
Antigens, Viral - immunology
Bacterial Outer Membrane Proteins - chemistry
Bacterial Outer Membrane Proteins - immunology
Bacterial Vaccines - administration & dosage
Bacterial Vaccines - genetics
Bacterial Vaccines - immunology
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fluorescence
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Hepacivirus - genetics
Hepatitis B Surface Antigens - genetics
ice nucleation protein
Immunoblotting
Immunogenicity
Immunoglobulin G - blood
Industrial applications and implications. Economical aspects
Infusions, Parenteral
Life Sciences
Liver - virology
Mice
Mice, Inbred BALB C
Mutagenesis
Nucleation
Production of active biomolecules
Pseudomonas syringae
Salmonella
Salmonella typhi
Salmonella typhi - immunology
Spleen - virology
Time Factors
Vaccines
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - genetics
Vaccines, Synthetic - immunology
Vaccins
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Summary:We have developed a recombinant live oral vaccine using the ice-nucleation protein (Inp) from Pseudomonas syringae to display viral antigens on the surface of Salmonella spp . Fusion proteins containing viral antigens were expressed in the oral vaccine strain, Salmonella typhi Ty21a. Surface localization was verified by immunoblotting and fluorescence-activated cell sorting. The immunogenicity of surface-displayed viral antigens on the recombinant live vaccine strain was assessed in mice inoculated intranasally and intraperitoneally. Inoculation resulted in significantly higher serum antibody level than those induced by viral antigens expressed intracellularly. Thus, this multivalent mucosal live vaccine may provide an effective means for inducing mucosal or systemic immune responses against multiple viral antigens.
ISSN:1087-0156
1546-1696
DOI:10.1038/76494