Effects of Repeated Chlamydia pneumoniae Inoculations on Aortic Lipid Accumulation and Inflammatory Response in C57BL/6J Mice

• Published in: Infection and Immunity 2005-10, Vol.73 (10), p.6458-6466
• Main Authors: Törmäkangas, Liisa, Erkkilä, Leena, Korhonen, Taina, Tiirola, Terttu, Bloigu, Aini, Saikku, Pekka, Leinonen, Maija
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Bacterial - blood; Arteriosclerosis - etiology; Arteriosclerosis - metabolism; Arteriosclerosis - microbiology; Bacterial Infections; Bacteriology; Biological and medical sciences; Chaperonin 60 - immunology; Chlamydophila Infections - immunology; Chlamydophila Infections - metabolism; Chlamydophila Infections - microbiology; Chlamydophila pneumoniae - pathogenicity; Cholesterol - blood; Cholesterol, Dietary - administration & dosage; DNA, Bacterial - analysis; Female; Fundamental and applied biological sciences. Psychology; Lipid Metabolism; Lipids - analysis; Lung - chemistry; Lung - pathology; Mice; Mice, Inbred C57BL; Microbiology; Miscellaneous; Pneumonia, Bacterial - metabolism; Pneumonia, Bacterial - microbiology; RNA, Bacterial - analysis; Sinus of Valsalva - chemistry; Sinus of Valsalva - metabolism; Sinus of Valsalva - pathology; Triglycerides - blood
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.73.10.6458-6466.2005

Chlamydia pneumoniae is a common respiratory tract pathogen, and persistent infections have been associated with atherosclerosis. We studied the effects of repeated chlamydial inoculations on the inflammatory response and on aortic lipid accumulation in C57BL/6J mice. Mice fed a diet supplemented with 0.2% cholesterol were infected three or six times with C. pneumoniae every fourth week. Sera and lungs were analyzed for inflammatory responses, lung tissues were tested for the presence of C. pneumoniae DNA and RNA, and intimal lipid accumulation in the aortic sinus was quantified. High levels of chlamydial heat shock protein 60 (Hsp60) immunoglobulin G2c subclass antibodies were detected in all of the infected mice, and a positive and statistically significant correlation was found between these antibodies and autoantibodies against mouse Hsp60. Both Hsp60 antibody levels correlated with the severity of lung tissue inflammation. The cholesterol supplement in the diet had no effect on serum cholesterol levels. Significantly larger intimal lipid lesions were seen in the mouse group infected six times (6,542 [micro]m²) than in the control group (1,376 [micro]m²; P = 0.034). In conclusion, repeated inoculations increased aortic sinus lipid accumulation in normocholesterolemic mice. The correlation between the antibodies to mouse and chlamydial Hsp60 proteins and their association with lung inflammation further support the theory of the development of an autoimmune response against heat shock proteins after repeated chlamydial infections.