In vitro regeneration through direct shoot organogenesis in Honey Orange (Citrus tangerina)

Rapid propagation of Honey orange (Citrus tangerina) was achieved by induction of shoots from epicotyl and cotyledonary node explants and rooting of cotyledonary node derived shoots. Significant explant differences were observed in the induction of direct shoots. Cotyledonary node explant is the mos...

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Bibliographic Details
Published in:Plant Biotechnology 2014/12/25, Vol.31(4), pp.341-344
Main Authors: Nwe, Yin Yin, Myint, Khin Thida, Mochizuki, Yuya, Vazirzanjani, Mehran, Okayasu, Kouji, Suzuki, Sakae, Ogiwara, Isao
Format: Article
Language:English
Subjects:
Citrus
Direct organogenesis
honey orange
seedling explants
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Summary:Rapid propagation of Honey orange (Citrus tangerina) was achieved by induction of shoots from epicotyl and cotyledonary node explants and rooting of cotyledonary node derived shoots. Significant explant differences were observed in the induction of direct shoots. Cotyledonary node explant is the most efficient in regeneration frequency followed by epicotyl explant. Cotyledonary node explants cultured on Murashige and Skoog (MS) medium supplemented with 8.88 µM N6-benzyladenine (BA) and 0.54 µM α-naphthaleneacetic acid (NAA) developed more than five shoots per explant. The isolated shoots transferred onto the MS medium supplemented with 5.4 µM NAA rooted 100% within 30 days.
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.14.0929a