Mitochondrial proteomes of porcine kidney cortex and medulla: foundation for translational proteomics

• Published in: Clinical and experimental nephrology 2016-02, Vol.20 (1), p.39-49
• Main Authors: Tuma, Zdenek, Kuncova, Jitka, Mares, Jan, Matejovic, Martin
• Format: Article
• Language: English
• Subjects: Animals; Electrophoresis, Gel, Two-Dimensional; Female; Kidney Cortex - chemistry; Kidney Medulla - chemistry; Male; Medicine; Medicine & Public Health; Mitochondria - chemistry; Mitochondrial Proteins - analysis; Models, Animal; Nephrology; Original Article; Proteomics - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sus scrofa; Tandem Mass Spectrometry; Translational Medical Research - methods; Urology
• ISSN: 1342-1751; 1437-7799
• DOI: 10.1007/s10157-015-1135-x

Background Emerging evidence has linked mitochondrial dysfunction to the pathogenesis of many renal disorders, including acute kidney injury, sepsis and even chronic kidney disease. Proteomics is a powerful tool in elucidating the role of mitochondria in renal pathologies. Since the pig is increasingly recognized as a major mammalian model for translational research, the lack of physiological proteome data of large mammals prompted us to examine renal mitochondrial proteome in porcine kidney cortex and medulla Methods Kidneys were obtained from six healthy pigs. Mitochondria from cortex and medulla were isolated using differential centrifugation and proteome maps of cortical and medullar mitochondria were constructed using two-dimensional gel electrophoresis (2DE). Protein spots with significant difference between mitochondrial fraction of renal cortex and medulla were identified by mass spectrometry. Results Proteomic analysis identified 81 protein spots. Of these spots, 41 mitochondrial proteins were statistically different between renal cortex and medulla ( p