Co-inoculation of Acacia mangium with Glomus intraradices and Bradyrhizobium sp. in aeroponic culture

• Published in: Biology and fertility of soils 2005-05, Vol.41 (4), p.233-239
• Main Authors: WEBER, Jean, DUCOUSSO, Marc, FOONG YEE THAM, NOURISSIER-MOUNTOU, Sophie, GALIANA, Antoine, PRIN, Yves, SING KONG LEE
• Format: Article
• Language: English
• Subjects: Aeroponics; Agronomy. Soil science and plant productions; Biological and medical sciences; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Immunization; Nutrient concentrations; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
• ISSN: 0178-2762; 1432-0789
• DOI: 10.1007/s00374-005-0833-z

Acacia mangium grown in aeroponic culture was co-inoculated with selected strains of Bradyrhizobium sp. and Glomus intraradices. A single-step technique using alginate as an embedding and sticking agent for an inoculum composed of arbuscular mycorrhiza (AM)-infected sheared roots was used to infect plants. This method resulted in the successful establishment of AM in 100% of the inoculated plants after 7 weeks. The results indicated that dual microbial inoculation with Glomus intraradices strain S-043 and Bradyrhizobium strain AUST 13C stimulated the growth of A. mangium in aeroponic culture. The effects of single and dual microbial inoculations were also evaluated at two levels of P in the nutrient medium. A concentration of 5 mg P kg^sup -1^ stimulated the development of AM without affecting plant development or establishment of Bradyrhizobium symbiosis. In contrast, saplings supplemented with a higher concentration of P (25 mg kg^sup -1^) alone or co-inoculated with Bradyrhizobium had lower AM frequencies.[PUBLICATION ABSTRACT]