In vitro Propagation of Limonium wrightii (Hance) Ktze. (Plumbaginaceae), an Ethnomedicinal Plant, from Shoot-Tip, Leaf- and Inflorescence-Node Explants

• Published in: In vitro cellular & developmental biology. Plant 2000-05, Vol.36 (3), p.220-224
• Main Authors: Huang, C. L., M. T. Hsieh, W. C. Hsieh, A. P. Sagare, H. S. Tsay
• Format: Article
• Language: English
• Subjects: 1-naphthyleneacetic acid; Adventitious shoots; Agronomy. Soil science and plant productions; Biological and medical sciences; Callus; Confidence limits; Economic plant physiology; Fundamental and applied biological sciences. Psychology; In vitro culture; Limonium wrightii; Medicinal plants; Micropropagation; N6-benzyladenine; Phytotrons; Plant cells; Plant physiology and development; Plant propagation; Plant roots; Plants; Plumbaginaceae; Rooting; Tissue cultures, protoplasts
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-000-0041-3

Rapid in vitro propagation of Limonium wrightii (Hance) Ktze. (Plumbaginaceae), an endangered medicinal plant, was achieved by culturing the shoot-tip (primary and lateral), leaf- and inflorescence-node Explants. MS (Murashige and Skoog, 1962) medium supplemented with$8.87 \mu M$ $N^6-benzyladenine$(BA) and$1.07 \umM$ $\alpha-naphthaleneacetic$acid (NAA) supported induction of adventitious shoots from the shoot-tip, inflorescence-node and middle and basal parts of leaf Explants after 60 d of culture. Adventitious shoots were multiplied by subculturing on MS medium supplemented with BA ($2.21-17.75 \mu M$) in combination with NAA ($1.07 \mu M$). The percentage of Explants forming shoots and the average number of adventitious shoot buds produced per Explant were stimulated by increasing the strength (1/4 x, 1/2 x, l x, 2 x) of the MS medium. Shoots were rooted on MS basal medium with$4.92 \mu M$indole-3-butyric acid. Plantlets with a morphologically normal appearance produced from adventitious shoots were transferred to soil and acclimated in the growth chamber for 1 mo.