Eukaryotic Hsp70 chaperones in the intermembrane space of chloroplasts

• Published in: Planta 2016-03, Vol.243 (3), p.733-747
• Main Authors: Bionda, Tihana, Gross, Lucia E, Becker, Thomas, Papasotiriou, Dimitrios G, Leisegang, Matthias S, Karas, Michael, Schleiff, Enrico
• Format: Article
• Language: English
• Subjects: Agriculture; Alfalfa; Arabidopsis - cytology; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis thaliana; Biomedical and Life Sciences; chloroplasts; Chloroplasts - metabolism; Cytosol - metabolism; Ecology; energy; Energy sources; Forestry; HSP70 Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins - isolation & purification; HSP70 Heat-Shock Proteins - metabolism; HSP90 Heat-Shock Proteins - genetics; HSP90 Heat-Shock Proteins - metabolism; Intracellular Membranes - metabolism; Life Sciences; Mass Spectrometry; Medicago sativa; Medicago sativa - cytology; Medicago sativa - genetics; Medicago sativa - metabolism; mitochondria; molecular chaperones; ORIGINAL ARTICLE; Pisum sativum - cytology; Pisum sativum - genetics; Pisum sativum - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; Protein Transport; Translocation
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-015-2440-z

MAIN CONCLUSION : Multiple eukaryotic Hsp70 typically localized in the cytoplasm are also distributed to the intermembrane space of chloroplasts and might thereby represent the missing link in energizing protein translocation. Protein translocation into organelles is a central cellular process that is tightly regulated. It depends on signals within the preprotein and on molecular machines catalyzing the process. Molecular chaperones participate in transport and translocation of preproteins into organelles to control folding and to provide energy for the individual steps. While most of the processes are explored and the components are identified, the transfer of preproteins into and across the intermembrane space of chloroplasts is not yet understood. The existence of an energy source in this compartment is discussed, because the required transit peptide length for successful translocation into chloroplasts is shorter than that found for mitochondria where energy is provided exclusively by matrix chaperones. Furthermore, a cytosolic-type Hsp70 homologue was proposed as component of the chloroplast translocon in the intermembrane space energizing the initial translocation. The molecular identity of such intermembrane space localized Hsp70 remained unknown, which led to a controversy concerning its existence. We identified multiple cytosolic Hsp70s by mass spectrometry on isolated, thermolysin-treated Medicago sativa chloroplasts. The localization of these Hsp70s of M. sativa or Arabidopsis thaliana in the intermembrane space was confirmed by a self-assembly GFP-based in vivo system. The localization of cytosolic Hsp70s in the stroma of chloroplasts or different mitochondrial compartments could not be observed. Similarly, we could not identify any cytosolic Hsp90 in the intermembrane space of chloroplast. With respect to our results we discuss the possible targeting and function of the Hsp70 found in the intermembrane space.