Defence responses against TNV infection induced by galactoglucomannan-derived oligosaccharides in cucumber cells

• Published in: European journal of plant pathology 2000-07, Vol.106 (6), p.543-553
• Main Authors: SLOVAKOVA, L, LISKOVA, D, CAPEK, P, KUBACKOVA, M, KAKONIOVA, D, KARACSONYI, S
• Format: Article
• Language: English
• Subjects: b-Glucanase; Biological and medical sciences; Cucumis sativus; Fundamental and applied biological sciences. Psychology; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control; Infections; Microbiology; oligosaccharides; Phytopathology. Animal pests. Plant and forest protection; Plant diseases; Plant viruses and viroids; Tobacco necrosis virus; Viral infections
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1023/A:1008722318178

Galactoglucomannan-derived oligosaccharides (GGMOs) showing biological activity in growth, morphogenesis and cell viability were tested in a host pathogen interaction. As a model system, cucumber (Cucumis sativus L. cv. Laura) reacting hypersensitively to tobacco necrosis virus (TNV) was used. The defence reactions were dependent on the degree of polymerisation and concentration of oligosaccharides, as well as on the time of application of virus to plant cotyledons. Disease symptoms were inhibited by 60-75%. The average number of lesions per cotyledon was significantly decreased when oligosaccharides were used simultaneously or 24h prior to virus inoculation. Significant changes in peroxidase, beta-glucanase and chitinase activities accompanied the defence reaction. It can be concluded that oligosaccharides derived from spruce galactoglucomannan induce non-specific resistance to local viral infection in plants. GGMOs probably act as inhibitors of the virus infection, rather than inhibitors of direct virus multiplication.[PUBLICATION ABSTRACT]