A new efficient expression system for Bacillus and its application to production of recombinant phytase

A new expression system was developed for Bacillus subtilis.This system uses a shuttle vector (B. subtilis- Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation als...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 2000-08, Vol.22 (16), p.1311-1317
Main Authors: KEROVUO, Janne, VON WEYMARN, Niklas, POVELAINEN, Mira, AUER, Sanna, MIASNIKOV, Andrei
Format: Article
Language:English
Subjects:
Bacillus subtilis
Biological and medical sciences
Biotechnology
Cultivation
Enzyme engineering
Escherichia coli
Fermentation
Fundamental and applied biological sciences. Psychology
Medical research
Methods. Procedures. Technologies
Microbiology
Production of selected enzymes
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new expression system was developed for Bacillus subtilis.This system uses a shuttle vector (B. subtilis- Eschericia coli) carrying a phosphate starvation-inducible promoter (pst) and on a fed-batch cultivation strategy. The pst-promoter proved to be very strong and retain its tight regulation also when present on a multi-copy plasmid. The expression system developed showed promising results when applied to the production of recombinant Bacillusphytase - phytase activity at the end of cultivation reached 28.7 U ml^sup -1^.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005694731039