Veratric Acid Inhibits LPS-Induced IL-6 and IL-8 Production in Human Gingival Fibroblasts

Veratric acid, one of the major benzoic acid isolated from vegetables and fruits, has been reported to have anti-inflammatory activity. The purpose of this study was to investigate the anti-inflammatory effects of veratric acid on lipopolysaccharide (LPS)-induced inflammatory response in human gingi...

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Bibliographic Details
Published in:Inflammation 2016-02, Vol.39 (1), p.237-242
Main Authors: Wang, Qi-bao, Sun, Lan-ying, Gong, Zuo-de, Du, Yi
Format: Article
Language:English
Subjects:
Anti-Inflammatory Agents - pharmacology
Biomedical and Life Sciences
Biomedicine
Cell Survival - drug effects
Cells, Cultured
Cyclooxygenase 2 - biosynthesis
Fibroblasts - cytology
Fibroblasts - immunology
Gingiva - cytology
Gingiva - immunology
Gingivitis - drug therapy
Gingivitis - immunology
Gingivitis - prevention & control
Humans
Immunology
Inflammation - chemically induced
Inflammation - drug therapy
Interleukin-6 - biosynthesis
Interleukin-8 - biosynthesis
Internal Medicine
Lipopolysaccharides
MAP Kinase Signaling System - drug effects
NF-kappa B - biosynthesis
Nitric Oxide Synthase Type II - biosynthesis
Pathology
Periodontitis - drug therapy
Periodontitis - pathology
Periodontitis - prevention & control
Pharmacology/Toxicology
Phosphatidylinositol 3-Kinases - biosynthesis
Proto-Oncogene Proteins c-akt - biosynthesis
Rheumatology
Vanillic Acid - analogs & derivatives
Vanillic Acid - pharmacology
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Summary:Veratric acid, one of the major benzoic acid isolated from vegetables and fruits, has been reported to have anti-inflammatory activity. The purpose of this study was to investigate the anti-inflammatory effects of veratric acid on lipopolysaccharide (LPS)-induced inflammatory response in human gingival fibroblasts (HGFs). HGFs were pretreated with veratric acid 1 h before LPS stimulation. The productions of IL-6 and IL-8 were detected by ELISA. The expression of iNOS, COX-2, PI3K, AKT, and NF-κB were detected by western blotting. The results showed that veratric acid inhibited LPS-induced IL-6 and IL-8 production, as well as iNOS and COX-2 expression. Veratric acid also inhibited LPS-induced NF-κB activation. In addition, veratric acid was found to suppress LPS-induced PI3K and AKT phosphorylation. In conclusion, the anti-inflammatory mechanism of veratric acid is due to its ability to inhibit PI3K/Akt/NF-κB signaling pathway.
ISSN:0360-3997
1573-2576
DOI:10.1007/s10753-015-0243-9