Effect of certain entomopathogenic fungi on oxidative stress and mortality of Periplaneta americana

• Published in: Pesticide biochemistry and physiology 2016-02, Vol.127, p.28-37
• Main Authors: Chaurasia, Abhilasha, Lone, Yaqoob, Wani, Owais, Gupta, U.S.
• Format: Article
• Language: English
• Subjects: Animals; Catalase; Daucus; Entomopathogenic fungi; Fungi - pathogenicity; Hirsutella; Lactate dehydrogenase; Lipid peroxidation; Metarhizium anisopliae; Mortality; Oxidative Stress; Periplaneta - metabolism; Periplaneta - microbiology; Periplaneta americana; Pest Control, Biological; Solanum tuberosum; Virulence
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2015.09.002

The present paper reports the effects of Metarhizium anisopliae, Isaria fumosoroseus and Hirsutella thompsonaii on Periplaneta americana. I. fumosoroseus and H. thompsonaii were cultured at 28±1°C on potato carrot agar and M. anisopliae was cultured at 28±1°C on potato dextrose agar for 14days. Conidial suspensions of fungi were given to cockroaches through different routes. M. anisopliae shows high virulence against adult cockroaches and mortality ranges from 38.65% to 78.36% after 48h. I. fumosoroseus and H. thompsonii show less virulence compared to M. anisopliae. We also investigated the effect of these three fungi on the activity of lactate dehydrogenase, lipid peroxidation and catalase in different tissues of the insect to gain an understanding of the different target site. The result suggested that the activity of lactate dehydrogenase, catalase and level of malondialdehyde varies in different organs and through different routes of exposure. Based on mortality percentages, all tested fungi had high potentials for biocontrol agents against P. americana. Our study reveals for the first time that I. fumosoroseus and H. thompsonaii fungal infections initiate oxidative stress in the midgut, fat body, whole body and hemolymph of cockroach thereby suggesting them to be the target organs for oxidative damage. [Display omitted] •Depending on the mode of administration, different entomopathogenic fungi caused up to 80% mortality•Entomopathogenic fungi modulated main antioxidant enzymes to enhance ROS level in midgut, fat body, whole body and hemolymph•Administration of entomopathogenic fungi altered the level of lipid peroxides both in tissues and hemolymph