Surface display of a borrelial lipoprotein on meningococcal outer membrane vesicles

Highlights • Borrelial lipoprotein OspA loses its outer-surface localization in Neisseria. • Surface-display rescued by fusion to part of meningococcal surface-lipoprotein fHbp. • OMVs with surface-exposed OspA elicit OspA-specific antibody responses in mice. • New method for surface display of hete...

Full description

Saved in:
Bibliographic Details
Published in:Vaccine 2016-02, Vol.34 (8), p.1025-1033
Main Authors: Salverda, Merijn L.M, Meinderts, Sanne M, Hamstra, Hendrik-Jan, Wagemakers, Alex, Hovius, Joppe W.R, van der Ark, Arno, Stork, Michiel, van der Ley, Peter
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antibodies, Bacterial - blood
Antigens
Antigens, Bacterial - immunology
Antigens, Surface - immunology
Bacteria
Bacterial Outer Membrane Proteins - immunology
Bacterial Proteins - immunology
Bacterial Vaccines - immunology
Bacteriology
Borrelia burgdorferi
Cloning
Conflicts of interest
Deoxyribonucleic acid
DNA
Female
fHbp
Gram-negative bacteria
Heterologous expression
Immune response
Immunization
Lipoprotein
Lipoproteins
Lipoproteins - immunology
Localization
Meningitis
Mice
Mice, Inbred BALB C
Neisseria meningitidis
Neisseria meningitidis - immunology
OMV
OspA
Outer Membrane Vesicles
Proteins
Surface display
Vaccines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Highlights • Borrelial lipoprotein OspA loses its outer-surface localization in Neisseria. • Surface-display rescued by fusion to part of meningococcal surface-lipoprotein fHbp. • OMVs with surface-exposed OspA elicit OspA-specific antibody responses in mice. • New method for surface display of heterologous lipoproteins in OMVs.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2016.01.019