High Gestational Folic Acid Supplementation Alters Expression of Imprinted and Candidate Autism Susceptibility Genes in a sex-Specific Manner in Mouse Offspring

Maternal nutrients play critical roles in modulating epigenetic events and exert long-term influences on the progeny’s health. Folic acid (FA) supplementation during pregnancy has decreased the incidence of neural tube defects in newborns, but the influence of high doses of maternal FA supplementati...

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Published in:Journal of molecular neuroscience 2016-02, Vol.58 (2), p.277-286
Main Authors: Barua, Subit, Kuizon, Salomon, Ted Brown, W., Junaid, Mohammed A.
Format: Article
Language:English
Subjects:
Animals
Autism
Autistic Disorder - genetics
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cerebral Cortex - drug effects
Cerebral Cortex - embryology
Cerebral Cortex - metabolism
Dietary Supplements
DNA Methylation
Female
Folic Acid - administration & dosage
Folic Acid - pharmacology
Genetic Predisposition to Disease
Genomic Imprinting
Male
Mice
Mice, Inbred C57BL
Neurochemistry
Neurology
Neurosciences
Pregnancy
Prenatal Exposure Delayed Effects - genetics
Prenatal Nutritional Physiological Phenomena - genetics
Proteomics
Sex Factors
Vitamin B Complex - administration & dosage
Vitamin B Complex - pharmacology
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description Maternal nutrients play critical roles in modulating epigenetic events and exert long-term influences on the progeny’s health. Folic acid (FA) supplementation during pregnancy has decreased the incidence of neural tube defects in newborns, but the influence of high doses of maternal FA supplementation on infants’ brain development is unclear. The present study was aimed at investigating the effects of a high dose of gestational FA on the expression of genes in the cerebral hemispheres (CHs) of 1-day-old pups. One week prior to mating and throughout the entire period of gestation, female C57BL/6J mice were fed a diet, containing FA at either 2 mg/kg (control diet (CD)) or 20 mg/kg (high maternal folic acid (HMFA)). At postnatal day 1, pups from different dams were sacrificed and CH tissues were collected. Quantitative RT-PCR and Western blot analysis confirmed sex-specific alterations in the expression of several genes that modulate various cellular functions ( P  
doi_str_mv 10.1007/s12031-015-0673-8
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Folic acid (FA) supplementation during pregnancy has decreased the incidence of neural tube defects in newborns, but the influence of high doses of maternal FA supplementation on infants’ brain development is unclear. The present study was aimed at investigating the effects of a high dose of gestational FA on the expression of genes in the cerebral hemispheres (CHs) of 1-day-old pups. One week prior to mating and throughout the entire period of gestation, female C57BL/6J mice were fed a diet, containing FA at either 2 mg/kg (control diet (CD)) or 20 mg/kg (high maternal folic acid (HMFA)). At postnatal day 1, pups from different dams were sacrificed and CH tissues were collected. Quantitative RT-PCR and Western blot analysis confirmed sex-specific alterations in the expression of several genes that modulate various cellular functions ( P  &lt; 0.05) in pups from the HMFA group. 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Folic acid (FA) supplementation during pregnancy has decreased the incidence of neural tube defects in newborns, but the influence of high doses of maternal FA supplementation on infants’ brain development is unclear. The present study was aimed at investigating the effects of a high dose of gestational FA on the expression of genes in the cerebral hemispheres (CHs) of 1-day-old pups. One week prior to mating and throughout the entire period of gestation, female C57BL/6J mice were fed a diet, containing FA at either 2 mg/kg (control diet (CD)) or 20 mg/kg (high maternal folic acid (HMFA)). At postnatal day 1, pups from different dams were sacrificed and CH tissues were collected. Quantitative RT-PCR and Western blot analysis confirmed sex-specific alterations in the expression of several genes that modulate various cellular functions ( P  &lt; 0.05) in pups from the HMFA group. 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Genomic DNA methylation analysis showed no difference in the level of overall methylation in pups from the HMFA group. These findings demonstrate that HMFA supplementation alters offsprings’ CH gene expression in a sex-specific manner. These changes may influence infants’ brain development.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26547318</pmid><doi>10.1007/s12031-015-0673-8</doi><tpages>10</tpages></addata></record>
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subjects Animals
Autism
Autistic Disorder - genetics
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cerebral Cortex - drug effects
Cerebral Cortex - embryology
Cerebral Cortex - metabolism
Dietary Supplements
DNA Methylation
Female
Folic Acid - administration & dosage
Folic Acid - pharmacology
Genetic Predisposition to Disease
Genomic Imprinting
Male
Mice
Mice, Inbred C57BL
Neurochemistry
Neurology
Neurosciences
Pregnancy
Prenatal Exposure Delayed Effects - genetics
Prenatal Nutritional Physiological Phenomena - genetics
Proteomics
Sex Factors
Vitamin B Complex - administration & dosage
Vitamin B Complex - pharmacology
title High Gestational Folic Acid Supplementation Alters Expression of Imprinted and Candidate Autism Susceptibility Genes in a sex-Specific Manner in Mouse Offspring
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