Aptamer-based colorimetric biosensing of abrin using catalytic gold nanoparticles

In this study we propose a simple and sensitive colorimetric aptasensor for the quantitative analysis of abrin by using catalytic AuNPs for the first time. AuNPs possess the peroxidase-like activity that can catalyse 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2, leading to color change...

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Bibliographic Details
Published in:Analyst (London) 2015-05, Vol.140 (10), p.3581-3586
Main Authors: Hu, Jingting, Ni, Pengjuan, Dai, Haichao, Sun, Yujing, Wang, Yilin, Jiang, Shu, Li, Zhuang
Format: Article
Language:English
Subjects:
Abrin - analysis
Abrin - chemistry
Animals
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - genetics
Base Sequence
Benzidines - chemistry
Biosensing Techniques - economics
Biosensing Techniques - methods
Catalysis
Catalysts
Cattle
Color
Colorimetry
Colorimetry - economics
Colorimetry - methods
Cost-Benefit Analysis
Gold - chemistry
Hydrogen Peroxide - chemistry
Hydrogen-Ion Concentration
Limit of Detection
Metal Nanoparticles - chemistry
Milk - chemistry
Nanoparticles
Quantitative analysis
Surface activation
Temperature
Thrombin
Time Factors
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Summary:In this study we propose a simple and sensitive colorimetric aptasensor for the quantitative analysis of abrin by using catalytic AuNPs for the first time. AuNPs possess the peroxidase-like activity that can catalyse 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2, leading to color change of the solution. It is interesting to find that the peroxidase-like activity of AuNPs can be improved by surface activation with a target-specific aptamer. However, with a target molecule, the aptamer is desorbed from the AuNPs surface, resulting in a decrease of the catalytic abilities of AuNPs. The color change of the solution is relevant to the target concentration, and this can be judged by the naked eye and monitored by using a UV-vis spectrometer. The linear range for the current analytical system was from 0.2 nM to 17.5 nM. The corresponding limit of detection (LOD) was 0.05 nM. Some other proteins such as thrombin (Th), glucose oxidase (GOx), and bovine serum albumin (BSA) all had a negligible effect on the determination of abrin. Furthermore, several practical samples spiked with abrin were analyzed using the proposed method with excellent recoveries. This aptamer-based colorimetric biosensor is superior to other conventional methods owing to its simplicity, low cost, and high sensitivity.
ISSN:0003-2654
1364-5528
DOI:10.1039/c5an00107b