The role of taurine on anxiety-like behaviors in zebrafish: A comparative study using the novel tank and the light–dark tasks

• Published in: Neuroscience letters 2016-02, Vol.613, p.19-24
• Main Authors: Mezzomo, Nathana J., Silveira, Ariane, Giuliani, Giulie S., Quadros, Vanessa A., Rosemberg, Denis B.
• Format: Article
• Language: English
• Subjects: Animals; Anxiety; Anxiety - psychology; Avoidance Learning - drug effects; Danio rerio; Darkness; Defensive behaviors; Light; Light–dark test; Motor Activity - drug effects; Novel tank; Taurine; Taurine - metabolism; Taurine - pharmacology; Zebrafish
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2015.12.037

•The effects of taurine were evaluated in zebrafish using two behavioral tasks.•Taurine did alter neither locomotor activity nor vertical exploration.•Taurine had anxiolytic-like effects in the light–dark test.•Behavioral endpoints are differently altered by acute taurine treatment. Taurine (TAU) is an amino sulfonic acid with several functions in central nervous system. Mounting evidence suggests that it acts in osmoregulation, neuromodulation and also as an inhibitory neurotransmitter. However, the effects of TAU on behavioral functions, especially on anxiety-related parameters, are limited. The adult zebrafish is a suitable model organism to examine anxiety-like behaviors since it presents neurotransmitter systems and behavioral functions evolutionary conserved. Anxiety in zebrafish can be measured by different tasks, analyzing the habituation to novelty, as well as the response to brightly lit environments. The aim of this study was to investigate whether acute TAU treatment alters anxiety-like behavior in zebrafish using the novel tank and the light-dark tests. Fish were individually treated with TAU (42, 150, and 400mg/L) for 1h and the behaviors were further analyzed for 6min in the novel tank or in the light–dark test. Control fish were handled in a similar manner, but kept only in home tank water. Although TAU did not alter locomotor and vertical activities, all concentrations significantly increased shuttling and time spent in lit compartment. Moreover, TAU 150 group showed a significant decrease in the number of risk assessment episodes. Overall, these data suggest that TAU exerts an anxiolytic-like effect in zebrafish and the comparative analysis of behavior using different tasks is an interesting strategy for neuropsychiatric studies related to anxiety in this species.