Production of sugars from macro-algae Gracilaria verrucosa using combined process of citric acid-catalyzed pretreatment and enzymatic hydrolysis

In this study, a combined process of citric acid-catalyzed pretreatment and enzymatic hydrolysis was applied for production of total reducing sugar (TRS) from marine macro red-algae Gracilaria verrucosa as a potential resource. By citric acid-catalyzed pretreatment under the 1:10 solid-to-liquid rat...

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Bibliographic Details
Published in:Algal research (Amsterdam) 2016-01, Vol.13, p.293-297
Main Authors: Kwon, Oh-Min, Kim, Dong-Hyun, Kim, Sung-Koo, Jeong, Gwi-Taek
Format: Article
Language:English
Subjects:
Citric acid-catalyzed pretreatment
Combined severity factor
Enzymatic hydrolysis
Gracilaria verrucosa
Total reducing sugar
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Summary:In this study, a combined process of citric acid-catalyzed pretreatment and enzymatic hydrolysis was applied for production of total reducing sugar (TRS) from marine macro red-algae Gracilaria verrucosa as a potential resource. By citric acid-catalyzed pretreatment under the 1:10 solid-to-liquid ratio, 0.1M citric acid, 150°C, 60min condition, a 50.9% TRS yield was obtained. By subsequent enzymatic hydrolysis using enzyme mixture (20 FBG/g-biomass and 28 FPU/g-biomass), a 57.8% TRS yield was obtained. Because citric acid-catalyzed pretreatment is simple, cheap, and environmentally friendly (do not use hazardous chemicals), these results served to demonstrate the significant potential of citric acid-catalyzed pretreatment to produce TRS for biofuels and biochemicals refinement purposes.
ISSN:2211-9264
2211-9264
DOI:10.1016/j.algal.2015.12.011