Oocyte maturation and expression pattern of follicular genes during in-vitro culture of vitrified mouse pre-antral follicles

Our aim was to evaluate the oocyte maturation rate and follicular genes expression pattern during in-vitro culture of vitrified mouse pre-antral follicles. Middle sized pre-antral follicles were isolated mechanically from the ovaries of pre-pubertal mice and distributed in vitrification and control...

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Bibliographic Details
Published in:Gene Expression Patterns 2016-01, Vol.20 (1), p.63-70
Main Authors: Jamalzaei, Parisa, Valojerdi, Mojtaba Rezazadeh, Ebrahimi, Bita, Farrokhi, Ali
Format: Article
Language:English
Subjects:
Animals
Cell Survival
Cryopreservation
Cryotop
Culture Media
Female
Follicular genes
Gene Expression Profiling
In-vitro culture
Mice
Oogenesis - genetics
Ovarian Follicle - cytology
Ovarian Follicle - embryology
Ovarian Follicle - metabolism
Pre-antral follicle
Tissue Culture Techniques - methods
Vitrification
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Summary:Our aim was to evaluate the oocyte maturation rate and follicular genes expression pattern during in-vitro culture of vitrified mouse pre-antral follicles. Middle sized pre-antral follicles were isolated mechanically from the ovaries of pre-pubertal mice and distributed in vitrification and control groups. In the vitrification group, follicles were washed in equilibration and vitrification solutions and then were immersed in liquid nitrogen after loading on cryotop tips. After warming in descending concentrations of sucrose solutions, fresh and vitrified-warmed follicles were cultured for 13 days. Follicles survival rate and follicular genes expression were assessed during in vitro culture. Finally, at the end of the culture period oocytes maturation rate were compared in both groups. In the vitrification group, follicles survival rate was lower significantly comparing to the control group (P 
ISSN:1567-133X
1872-7298
DOI:10.1016/j.gep.2015.12.001