Guggulsterone Attenuated Lipopolysaccharide-Induced Inflammatory Responses in Mouse Inner Medullary Collecting Duct-3 Cells

Guggulsterone (GS) is a phytosterol that has been used to treat inflammatory diseases such as colitis, obesity, and thrombosis. Although many previous studies have examined activities of GS, the effect of GS on lipopolysaccharide (LPS)-induced inflammatory responses in mouse inner medullary collecti...

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Bibliographic Details
Published in:Inflammation 2016-02, Vol.39 (1), p.87-95
Main Authors: Kim, Dong-Goo, Bae, Gi-Sang, Jo, Il-Joo, Choi, Sun-Bok, Kim, Myoung-Jin, Jeong, Jun-Hyeok, Kang, Dae-Gil, Lee, Ho-Sub, Song, Ho-Joon, Park, Sung-Joo
Format: Article
Language:English
Subjects:
Acute Kidney Injury - drug therapy
Animals
Anti-Inflammatory Agents - pharmacology
Biomedical and Life Sciences
Biomedicine
Cell Line
Cyclooxygenase 2 - biosynthesis
Female
Immunology
Inflammation - drug therapy
Interleukin-6 - biosynthesis
Internal Medicine
Kidney Tubules, Collecting - cytology
Kidney Tubules, Collecting - immunology
Lipopolysaccharides
Macrophages, Peritoneal - immunology
Mice
Mice, Inbred C57BL
NF-KappaB Inhibitor alpha - metabolism
Nitric Oxide Synthase Type II - biosynthesis
Pathology
Pharmacology/Toxicology
Pregnenediones - pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Rheumatology
Toll-Like Receptor 4 - immunology
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:Guggulsterone (GS) is a phytosterol that has been used to treat inflammatory diseases such as colitis, obesity, and thrombosis. Although many previous studies have examined activities of GS, the effect of GS on lipopolysaccharide (LPS)-induced inflammatory responses in mouse inner medullary collecting duct-3 (mIMCD-3) cells have not been examined. Therefore, here, we investigated the anti-inflammatory action of GS on mIMCD-3 cells exposed to LPS. LPS treatment on mIMCD-3 cells produced pro-inflammatory molecules such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) significantly; however, GS treatment significantly inhibited the production of pro-inflammatory molecules. In addition, GS inhibited the degradation of Iκ-Bα and translocation of NF-κB on mIMCD-3 cells. These results suggest that GS could inhibit inflammatory responses in collecting duct cells which could contribute to kidney injury during systemic infection.
ISSN:0360-3997
1573-2576
DOI:10.1007/s10753-015-0226-x