Toxoplasma gondii Infection Promotes Neuroinflammation Through Cytokine Networks and Induced Hyperalgesia in BALB/c Mice

We hypothesized that in Toxoplasma gondii infection, communication among immune cells promotes neuroinflammation through cytokine networks and induces pain sensitivity under conditions of neuropathic pain. The animal model of Toxoplasma infection was established by the intraperitoneal inoculation of...

Full description

Saved in:
Bibliographic Details
Published in:Inflammation 2016-02, Vol.39 (1), p.405-412
Main Authors: Mahmoudvand, Hossein, Ziaali, Naser, Ghazvini, Hamed, Shojaee, Saeideh, Keshavarz, Hossein, Esmaeilpour, Khadijeh, Sheibani, Vahid
Format: Article
Language:English
Subjects:
Amitriptyline - therapeutic use
Analgesics, Non-Narcotic - therapeutic use
Animals
Biomedical and Life Sciences
Biomedicine
Cytokines - immunology
Disease Models, Animal
Hyperalgesia - drug therapy
Hyperalgesia - parasitology
Immunology
Inflammation - immunology
Interleukin-1beta - genetics
Interleukin-6 - genetics
Internal Medicine
Male
Mice
Mice, Inbred BALB C
Neuralgia - drug therapy
Neuralgia - parasitology
Original Article
Pathology
Pharmacology/Toxicology
Real-Time Polymerase Chain Reaction
Rheumatology
RNA, Messenger - genetics
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis - parasitology
Toxoplasmosis - pathology
Tumor Necrosis Factor-alpha - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We hypothesized that in Toxoplasma gondii infection, communication among immune cells promotes neuroinflammation through cytokine networks and induces pain sensitivity under conditions of neuropathic pain. The animal model of Toxoplasma infection was established by the intraperitoneal inoculation of 20–25 tissue cysts from Tehran strain of T. gondii to BALB/c mice. Amitriptyline (20 mg/kg, i.p., 1/day) administrated to animals for 7 days before behavioral tests. Pain behavioral tests including tail flick, hot plate, and formalin test were evaluated in all the groups. The mRNA levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were examined by real-time PCR. Results revealed that T. gondii induce hyperalgesia in the infected mice, whereas amitriptyline showed a promising effect against the hyperalgesia induced by Toxoplasma infection. The mRNA levels of the aforementioned cytokines significantly ( P  
ISSN:0360-3997
1573-2576
DOI:10.1007/s10753-015-0262-6