Comparative studies on proliferation, molecular markers and differentiation potential of mesenchymal stem cells from various tissues (adipose, bone marrow, ear skin, abdominal skin, and lung) and maintenance of multipotency during serial passages in miniature pig

•The MSCs were isolated from various tissues of miniature pigs.•The proliferation of MSCs derived from all tissues was increased during 14 passages.•The molecular phenotype of mpMSCs was different during sub-passaging.•Differentiation potential was lost during sequential sub-passages.•Expression of...

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Bibliographic Details
Published in:Research in veterinary science 2015-06, Vol.100, p.115-124
Main Authors: Lee, Ah-Young, Lee, Jienny, Kim, Chan-Lan, Lee, Keum Sil, Lee, So-Hyun, Gu, Na-Yeon, Kim, Jeong-Min, Lee, Byeong Chun, Koo, Ok Jae, Song, Jae-Young, Cha, Sang-Ho
Format: Article
Language:English
Subjects:
Animals
Biomarkers
Bone marrow
Cell Differentiation
Cell Proliferation
Differentiation
Medical research
Mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Miniature pig
Multipotency
Organ Specificity
Proliferation
Serial Passage - veterinary
Stem cells
Studies
Swine
Swine, Miniature
Veterinary medicine
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Summary:•The MSCs were isolated from various tissues of miniature pigs.•The proliferation of MSCs derived from all tissues was increased during 14 passages.•The molecular phenotype of mpMSCs was different during sub-passaging.•Differentiation potential was lost during sequential sub-passages.•Expression of pluripotency genes of mpMSCs was different during sequential passaging. Mesenchymal stem cells (MSCs) have the ability to differentiate into multi-lineage cells, which confers great promise for use in regenerative medicine. In this study, MSCs were isolated from adipose tissue, bone marrow, ear skin, lung, and abdominal skin of miniature pigs (mpMSCs), and the optimal medium (DMEM/F12-Glutamax) was selected for the culturing of mpMSCs. As a result, proliferation of the mpMSCs derived from all tissues was steadily increased when cultured with DMEM/F12-Glutamax during 14 consecutive passages. The cells harbored MSC surface markers (CD34-, CD45-, CD29+, CD44+, CD90+, and CD105+), whose levels of expression differed among the tissue sources and declined over sub-passaging. In addition, the expression of stemness markers (Oct4, Sox2, and Nanog) and differentiation into mesoderm (adipocytes, chondrocytes, and osteoblasts) were clearly represented at early passage; however, expression of stemness markers decreased, and differentiation potential was lost over sequential sub-passaging, which should be considered in the selection of mpMSC for MSC-based application.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2015.03.010