Expression of a fusion protein of Pyrus pyrifolia S-RNase with glutathione-S-transferase in E. coli

S-RNase is a style-specific ribonuclease which is associated with gametophytic self-incompatibility. An expression vector of a fusion protein of Pyrus pyrifolia(Japanese pear) S3-RNase with glutathione-S-transferase (GST) was constructed and transformed into E. coli. Using this system, the fusion pr...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 2000-09, Vol.22 (17), p.1413-1417
Main Authors: GAMAGE, Niranjali, MATSUURA, Takanori, NORIOKA, Naoko, YOSHIMURA, Yumi, TAKASAKI, Takeshi, NAKANISHI, Tetsu, NORIOKA, Shigemi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
E coli
Escherichia coli
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Others
Pollen
Proteins
Pyrus pyrifolia
ribonuclease
Various methods and equipments
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:S-RNase is a style-specific ribonuclease which is associated with gametophytic self-incompatibility. An expression vector of a fusion protein of Pyrus pyrifolia(Japanese pear) S3-RNase with glutathione-S-transferase (GST) was constructed and transformed into E. coli. Using this system, the fusion protein, GST-S3-RNase, was expressed as an active form and can be used for screening pollen S-gene product(s).[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005617000553