Investigation of luminescent banding in solid coral : the contribution of phosphorescence

• Published in: Coral reefs 2000-07, Vol.19 (2), p.132-140
• Main Authors: WILD, F. J, JONES, A. C, TUDHOPE, A. W
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; General aspects. Techniques; Marine; Methods and techniques (sampling, tagging, trapping, modelling...); Porites
• ISSN: 0722-4028; 1432-0975
• DOI: 10.1007/s003380000084

This study investigates the nature and components of annual luminescent banding in massive Porites coral skeletons, with a view to refining the technique for using this banding to reconstruct past environmental conditions. Three-dimensional excitation-emission-matrix spectroscopy and optical fibre beam delivery have been used to investigate the luminescence properties of the bright and dull bands of solid coral. Six characteristic excitation/emission peaks have been identified: 280/450-600, 340 /450, 370/470, 390/485, 420/505 and 450/530 nm. The first peak corresponds to protein-type fluorescence. The others are characteristic of humic acid luminescence. The difference in luminescence intensity between bright and dull bands has been quantified and characterised spectroscopically. The luminescence of the bright bands is up to 25% more intense than their neighbouring dull bands with the greatest increase in relative intensity in the long wavelength emission region, between 500 and 600 nm. The contribution of long-lived phosphorescence to the total luminescence intensity has been determined by time-resolved measurements on the 100 ms timescale. Both bright and dull bands show long-lived phosphorescence with decay times up to 1.5 s. This phosphorescence accounts for about 10% of the total luminescence intensity of bright bands. The difference in phosphorescence intensity between bright and dull bands is substantially greater than the difference in total luminescence intensity: the phosphorescence of bright bands is up to twice as intense as that of dull bands. This suggests that phosphorescence plays an important role in defining luminescent banding in coral. Furthermore, the large observable difference in phosphorescence between bright and dull bands indicates that measurement of phosphorescence profiles across growth bands in corals may prove to be a more sensitive indicator of past environmental conditions than measurements of total luminescence.