In vitro effects of β-hydroxy-β-methylbutyrate (HMB) on cell-mediated immunity in fish

β-Hydroxy-β-methyl butyrate(HMB) has been shown to counteract many of the negative effects of intensive animal production methods and results in increased growth and protection against diseases. In the present study, the effect of HMB on the immunocompetence cell activity in rainbow trout ( Oncorhyn...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2000-10, Vol.76 (3), p.191-197
Main Authors: Siwicki, Andrzej K, Fuller, John C, Nissen, Steven, Ostaszewski, Piotr, Studnicka, Maria
Format: Article
Language:English
Subjects:
Animals
beta -hydroxy- beta -methylbutyric acid
Carps - immunology
Cell Division - drug effects
Cell Division - immunology
Concanavalin A - immunology
Cyprinus carpio
Fish
Formazans - chemistry
Freshwater
Immunity, Cellular - drug effects
Immunocompetence
Lipopolysaccharides - immunology
Lymphocyte Activation - drug effects
Lymphocyte Activation - immunology
Lymphocytes
Lymphocytes - drug effects
Lymphocytes - immunology
Macrophages
Oncorhynchus mykiss
Oncorhynchus mykiss - immunology
Phagocytes - drug effects
Phagocytes - immunology
Phagocytosis - drug effects
Phagocytosis - immunology
Respiratory Burst - drug effects
Respiratory Burst - immunology
Tetradecanoylphorbol Acetate - immunology
Tetrazolium Salts - chemistry
Valerates - immunology
Valerates - pharmacology
β-Hydroxy-β-methyl butyrate (HMB)
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Summary:β-Hydroxy-β-methyl butyrate(HMB) has been shown to counteract many of the negative effects of intensive animal production methods and results in increased growth and protection against diseases. In the present study, the effect of HMB on the immunocompetence cell activity in rainbow trout ( Oncorhynchus mykiss) and carp ( Cyprinus carpio) was examined. Pronephric phagocytes and lymphocytes were isolated from the fish and grown in culture medium (RPMI-1640) containing either 0, 0.1, 1, 5, 10, 25, 50 or 100 μg HMB/ml of medium. The effects of HMB on the respiratory burst activity (RBA) stimulated by phorbol myristate acetate (PMA), the potential killing activity (PKA) and lymphocyte proliferation stimulated by either concanavalin A (Con-A) or lipopolysaccharide (LPS) were examined. The addition of HMB to the culture medium increased the RBA by up to 84% ( p
ISSN:0165-2427
1873-2534
DOI:10.1016/S0165-2427(00)00211-7