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In vitro effects of β-hydroxy-β-methylbutyrate (HMB) on cell-mediated immunity in fish
β-Hydroxy-β-methyl butyrate(HMB) has been shown to counteract many of the negative effects of intensive animal production methods and results in increased growth and protection against diseases. In the present study, the effect of HMB on the immunocompetence cell activity in rainbow trout ( Oncorhyn...
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| Published in: | Veterinary immunology and immunopathology 2000-10, Vol.76 (3), p.191-197 |
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| cites | cdi_FETCH-LOGICAL-c392t-78501f8c6cb0cf2cf9667b88ae2f88b07edbf3f825fec27685e49085c2fbefba3 |
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| container_issue | 3 |
| container_start_page | 191 |
| container_title | Veterinary immunology and immunopathology |
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| creator | Siwicki, Andrzej K Fuller, John C Nissen, Steven Ostaszewski, Piotr Studnicka, Maria |
| description | β-Hydroxy-β-methyl butyrate(HMB) has been shown to counteract many of the negative effects of intensive animal production methods and results in increased growth and protection against diseases. In the present study, the effect of HMB on the immunocompetence cell activity in rainbow trout (
Oncorhynchus mykiss) and carp (
Cyprinus carpio) was examined. Pronephric phagocytes and lymphocytes were isolated from the fish and grown in culture medium (RPMI-1640) containing either 0, 0.1, 1, 5, 10, 25, 50 or 100
μg HMB/ml of medium. The effects of HMB on the respiratory burst activity (RBA) stimulated by phorbol myristate acetate (PMA), the potential killing activity (PKA) and lymphocyte proliferation stimulated by either concanavalin A (Con-A) or lipopolysaccharide (LPS) were examined. The addition of HMB to the culture medium increased the RBA by up to 84% (
p |
| doi_str_mv | 10.1016/S0165-2427(00)00211-7 |
| format | article |
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Oncorhynchus mykiss) and carp (
Cyprinus carpio) was examined. Pronephric phagocytes and lymphocytes were isolated from the fish and grown in culture medium (RPMI-1640) containing either 0, 0.1, 1, 5, 10, 25, 50 or 100
μg HMB/ml of medium. The effects of HMB on the respiratory burst activity (RBA) stimulated by phorbol myristate acetate (PMA), the potential killing activity (PKA) and lymphocyte proliferation stimulated by either concanavalin A (Con-A) or lipopolysaccharide (LPS) were examined. The addition of HMB to the culture medium increased the RBA by up to 84% (
p<0.01) over that of cells grown without HMB. Similarly, the PKA of the phagocytes was also increased with HMB addition to the medium by up to 140% (
p<0.01) over that of cells grown without HMB. Lymphocyte proliferation stimulated by both ConA and LPS was also increased approximately two-fold (
p<0.01) when HMB was added to the culture medium at concentrations between 10 and 100
μg HMB/ml in both rainbow trout and carp. The greatest effects of HMB on RBA and PKA activities were observed at a concentration >50
μg HMB/ml while lymphocyte proliferation was maximally stimulated at 25
μg HMB/ml. In conclusion, the current study shows that HMB could potentially improve immunocompetence cell activity in fish through increased cell proliferation and functionality.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/S0165-2427(00)00211-7</identifier><identifier>PMID: 11044553</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; beta -hydroxy- beta -methylbutyric acid ; Carps - immunology ; Cell Division - drug effects ; Cell Division - immunology ; Concanavalin A - immunology ; Cyprinus carpio ; Fish ; Formazans - chemistry ; Freshwater ; Immunity, Cellular - drug effects ; Immunocompetence ; Lipopolysaccharides - immunology ; Lymphocyte Activation - drug effects ; Lymphocyte Activation - immunology ; Lymphocytes ; Lymphocytes - drug effects ; Lymphocytes - immunology ; Macrophages ; Oncorhynchus mykiss ; Oncorhynchus mykiss - immunology ; Phagocytes - drug effects ; Phagocytes - immunology ; Phagocytosis - drug effects ; Phagocytosis - immunology ; Respiratory Burst - drug effects ; Respiratory Burst - immunology ; Tetradecanoylphorbol Acetate - immunology ; Tetrazolium Salts - chemistry ; Valerates - immunology ; Valerates - pharmacology ; β-Hydroxy-β-methyl butyrate (HMB)</subject><ispartof>Veterinary immunology and immunopathology, 2000-10, Vol.76 (3), p.191-197</ispartof><rights>2000 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-78501f8c6cb0cf2cf9667b88ae2f88b07edbf3f825fec27685e49085c2fbefba3</citedby><cites>FETCH-LOGICAL-c392t-78501f8c6cb0cf2cf9667b88ae2f88b07edbf3f825fec27685e49085c2fbefba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11044553$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Siwicki, Andrzej K</creatorcontrib><creatorcontrib>Fuller, John C</creatorcontrib><creatorcontrib>Nissen, Steven</creatorcontrib><creatorcontrib>Ostaszewski, Piotr</creatorcontrib><creatorcontrib>Studnicka, Maria</creatorcontrib><title>In vitro effects of β-hydroxy-β-methylbutyrate (HMB) on cell-mediated immunity in fish</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>β-Hydroxy-β-methyl butyrate(HMB) has been shown to counteract many of the negative effects of intensive animal production methods and results in increased growth and protection against diseases. In the present study, the effect of HMB on the immunocompetence cell activity in rainbow trout (
Oncorhynchus mykiss) and carp (
Cyprinus carpio) was examined. Pronephric phagocytes and lymphocytes were isolated from the fish and grown in culture medium (RPMI-1640) containing either 0, 0.1, 1, 5, 10, 25, 50 or 100
μg HMB/ml of medium. The effects of HMB on the respiratory burst activity (RBA) stimulated by phorbol myristate acetate (PMA), the potential killing activity (PKA) and lymphocyte proliferation stimulated by either concanavalin A (Con-A) or lipopolysaccharide (LPS) were examined. The addition of HMB to the culture medium increased the RBA by up to 84% (
p<0.01) over that of cells grown without HMB. Similarly, the PKA of the phagocytes was also increased with HMB addition to the medium by up to 140% (
p<0.01) over that of cells grown without HMB. Lymphocyte proliferation stimulated by both ConA and LPS was also increased approximately two-fold (
p<0.01) when HMB was added to the culture medium at concentrations between 10 and 100
μg HMB/ml in both rainbow trout and carp. The greatest effects of HMB on RBA and PKA activities were observed at a concentration >50
μg HMB/ml while lymphocyte proliferation was maximally stimulated at 25
μg HMB/ml. In conclusion, the current study shows that HMB could potentially improve immunocompetence cell activity in fish through increased cell proliferation and functionality.</description><subject>Animals</subject><subject>beta -hydroxy- beta -methylbutyric acid</subject><subject>Carps - immunology</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - immunology</subject><subject>Concanavalin A - immunology</subject><subject>Cyprinus carpio</subject><subject>Fish</subject><subject>Formazans - chemistry</subject><subject>Freshwater</subject><subject>Immunity, Cellular - drug effects</subject><subject>Immunocompetence</subject><subject>Lipopolysaccharides - immunology</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocytes</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - immunology</subject><subject>Macrophages</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - immunology</subject><subject>Phagocytes - drug effects</subject><subject>Phagocytes - immunology</subject><subject>Phagocytosis - drug effects</subject><subject>Phagocytosis - immunology</subject><subject>Respiratory Burst - drug effects</subject><subject>Respiratory Burst - immunology</subject><subject>Tetradecanoylphorbol Acetate - immunology</subject><subject>Tetrazolium Salts - chemistry</subject><subject>Valerates - immunology</subject><subject>Valerates - pharmacology</subject><subject>β-Hydroxy-β-methyl butyrate (HMB)</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkMFOxCAQQInRuOvqJ2g4GfdQHaAUejJq1DXReFATb6SlkMVsW4XW2N_yQ_wmWXejRy_DBN4wMw-hfQLHBEh28hADT2hKxRHAFIASkogNNCZSsIRylm6i8S8yQjshvAAAz6XcRiNCIE05Z2P0fNPgd9f5Fhtrje4Cbi3--kzmQ-XbjyGJaW26-bAo-27wRWfw0ezufIrbBmuzWMTHysXbCru67hvXDdg12Low30VbtlgEs7c-J-jp6vLxYpbc3l_fXJzdJprltEuE5ECs1JkuQVuqbZ5lopSyMNRKWYIwVWmZlZTH6ajIJDdpDpJraktjy4JN0OHq31ffvvUmdKp2YTla0Zi2D4qILGecsQjyFah9G4I3Vr16Vxd-UATUUqn6UaqWvhSA-lGqRKw7WDfoy7jtX9XaYQROV4CJa74741XQzjQ6mvHRqKpa90-Lbykih8g</recordid><startdate>20001031</startdate><enddate>20001031</enddate><creator>Siwicki, Andrzej K</creator><creator>Fuller, John C</creator><creator>Nissen, Steven</creator><creator>Ostaszewski, Piotr</creator><creator>Studnicka, Maria</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20001031</creationdate><title>In vitro effects of β-hydroxy-β-methylbutyrate (HMB) on cell-mediated immunity in fish</title><author>Siwicki, Andrzej K ; Fuller, John C ; Nissen, Steven ; Ostaszewski, Piotr ; Studnicka, Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-78501f8c6cb0cf2cf9667b88ae2f88b07edbf3f825fec27685e49085c2fbefba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>beta -hydroxy- beta -methylbutyric acid</topic><topic>Carps - immunology</topic><topic>Cell Division - drug effects</topic><topic>Cell Division - immunology</topic><topic>Concanavalin A - immunology</topic><topic>Cyprinus carpio</topic><topic>Fish</topic><topic>Formazans - chemistry</topic><topic>Freshwater</topic><topic>Immunity, Cellular - drug effects</topic><topic>Immunocompetence</topic><topic>Lipopolysaccharides - immunology</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocytes</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - immunology</topic><topic>Macrophages</topic><topic>Oncorhynchus mykiss</topic><topic>Oncorhynchus mykiss - immunology</topic><topic>Phagocytes - drug effects</topic><topic>Phagocytes - immunology</topic><topic>Phagocytosis - drug effects</topic><topic>Phagocytosis - immunology</topic><topic>Respiratory Burst - drug effects</topic><topic>Respiratory Burst - immunology</topic><topic>Tetradecanoylphorbol Acetate - immunology</topic><topic>Tetrazolium Salts - chemistry</topic><topic>Valerates - immunology</topic><topic>Valerates - pharmacology</topic><topic>β-Hydroxy-β-methyl butyrate (HMB)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Siwicki, Andrzej K</creatorcontrib><creatorcontrib>Fuller, John C</creatorcontrib><creatorcontrib>Nissen, Steven</creatorcontrib><creatorcontrib>Ostaszewski, Piotr</creatorcontrib><creatorcontrib>Studnicka, Maria</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Siwicki, Andrzej K</au><au>Fuller, John C</au><au>Nissen, Steven</au><au>Ostaszewski, Piotr</au><au>Studnicka, Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro effects of β-hydroxy-β-methylbutyrate (HMB) on cell-mediated immunity in fish</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2000-10-31</date><risdate>2000</risdate><volume>76</volume><issue>3</issue><spage>191</spage><epage>197</epage><pages>191-197</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>β-Hydroxy-β-methyl butyrate(HMB) has been shown to counteract many of the negative effects of intensive animal production methods and results in increased growth and protection against diseases. In the present study, the effect of HMB on the immunocompetence cell activity in rainbow trout (
Oncorhynchus mykiss) and carp (
Cyprinus carpio) was examined. Pronephric phagocytes and lymphocytes were isolated from the fish and grown in culture medium (RPMI-1640) containing either 0, 0.1, 1, 5, 10, 25, 50 or 100
μg HMB/ml of medium. The effects of HMB on the respiratory burst activity (RBA) stimulated by phorbol myristate acetate (PMA), the potential killing activity (PKA) and lymphocyte proliferation stimulated by either concanavalin A (Con-A) or lipopolysaccharide (LPS) were examined. The addition of HMB to the culture medium increased the RBA by up to 84% (
p<0.01) over that of cells grown without HMB. Similarly, the PKA of the phagocytes was also increased with HMB addition to the medium by up to 140% (
p<0.01) over that of cells grown without HMB. Lymphocyte proliferation stimulated by both ConA and LPS was also increased approximately two-fold (
p<0.01) when HMB was added to the culture medium at concentrations between 10 and 100
μg HMB/ml in both rainbow trout and carp. The greatest effects of HMB on RBA and PKA activities were observed at a concentration >50
μg HMB/ml while lymphocyte proliferation was maximally stimulated at 25
μg HMB/ml. In conclusion, the current study shows that HMB could potentially improve immunocompetence cell activity in fish through increased cell proliferation and functionality.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>11044553</pmid><doi>10.1016/S0165-2427(00)00211-7</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals beta -hydroxy- beta -methylbutyric acid Carps - immunology Cell Division - drug effects Cell Division - immunology Concanavalin A - immunology Cyprinus carpio Fish Formazans - chemistry Freshwater Immunity, Cellular - drug effects Immunocompetence Lipopolysaccharides - immunology Lymphocyte Activation - drug effects Lymphocyte Activation - immunology Lymphocytes Lymphocytes - drug effects Lymphocytes - immunology Macrophages Oncorhynchus mykiss Oncorhynchus mykiss - immunology Phagocytes - drug effects Phagocytes - immunology Phagocytosis - drug effects Phagocytosis - immunology Respiratory Burst - drug effects Respiratory Burst - immunology Tetradecanoylphorbol Acetate - immunology Tetrazolium Salts - chemistry Valerates - immunology Valerates - pharmacology β-Hydroxy-β-methyl butyrate (HMB) |
| title | In vitro effects of β-hydroxy-β-methylbutyrate (HMB) on cell-mediated immunity in fish |
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